Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems

ABSTRACT: Cryptosporidiosis is considered an infection with impact on animal health. It has been associated with high morbidity and mortality rates, leading to significant economic losses to the poultry industry. This study investigated the presence of Cryptosporidium spp. in domestic ducks of family Anatidae (Cairina moschata) from two rustic commercial aviaries located in the city of Rio de Janeiro, Brazil. A total of 315 fecal samples were collected from domestic ducks in two different areas (N=186 in area A and N=129 in area B). The microscopic analysis was conducted using a sugar centrifugal flotation technique for the identification of Cryptosporidium spp. oocysts, followed by PCR/sequencing analyses of the partial sequence of the 18S rDNA gene to determine the Cryptosporidium species. Of the 315 samples collected, only 10 (186/5.38%) from area A were positive for Cryptosporidium. The nucleotide sequence and phylogenetic analyses identified that all samples were identical (100%) and belonged to Cryptosporidium baileyi species, which is closely related to gastric species and of importance in animal health.

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Main Authors: Berriel,Lidiane Tavares Duarte, Sudré,Adriana Pittella, Santos,Helena Lúcia Carneiro, Bomfim,Teresa Cristina Bergamo do
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000500501
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spelling oai:scielo:S0103-847820220005005012021-10-27Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systemsBerriel,Lidiane Tavares DuarteSudré,Adriana PittellaSantos,Helena Lúcia CarneiroBomfim,Teresa Cristina Bergamo do muscovy ducks protozoan molecular analysis Cryptosporidium baileyi ABSTRACT: Cryptosporidiosis is considered an infection with impact on animal health. It has been associated with high morbidity and mortality rates, leading to significant economic losses to the poultry industry. This study investigated the presence of Cryptosporidium spp. in domestic ducks of family Anatidae (Cairina moschata) from two rustic commercial aviaries located in the city of Rio de Janeiro, Brazil. A total of 315 fecal samples were collected from domestic ducks in two different areas (N=186 in area A and N=129 in area B). The microscopic analysis was conducted using a sugar centrifugal flotation technique for the identification of Cryptosporidium spp. oocysts, followed by PCR/sequencing analyses of the partial sequence of the 18S rDNA gene to determine the Cryptosporidium species. Of the 315 samples collected, only 10 (186/5.38%) from area A were positive for Cryptosporidium. The nucleotide sequence and phylogenetic analyses identified that all samples were identical (100%) and belonged to Cryptosporidium baileyi species, which is closely related to gastric species and of importance in animal health.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.52 n.5 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000500501en10.1590/0103-8478cr20210001
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Berriel,Lidiane Tavares Duarte
Sudré,Adriana Pittella
Santos,Helena Lúcia Carneiro
Bomfim,Teresa Cristina Bergamo do
spellingShingle Berriel,Lidiane Tavares Duarte
Sudré,Adriana Pittella
Santos,Helena Lúcia Carneiro
Bomfim,Teresa Cristina Bergamo do
Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems
author_facet Berriel,Lidiane Tavares Duarte
Sudré,Adriana Pittella
Santos,Helena Lúcia Carneiro
Bomfim,Teresa Cristina Bergamo do
author_sort Berriel,Lidiane Tavares Duarte
title Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems
title_short Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems
title_full Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems
title_fullStr Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems
title_full_unstemmed Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems
title_sort molecular identification of cryptosporidium baileyi in muscovy ducks (cairina moschata domesticus) in free-range production systems
description ABSTRACT: Cryptosporidiosis is considered an infection with impact on animal health. It has been associated with high morbidity and mortality rates, leading to significant economic losses to the poultry industry. This study investigated the presence of Cryptosporidium spp. in domestic ducks of family Anatidae (Cairina moschata) from two rustic commercial aviaries located in the city of Rio de Janeiro, Brazil. A total of 315 fecal samples were collected from domestic ducks in two different areas (N=186 in area A and N=129 in area B). The microscopic analysis was conducted using a sugar centrifugal flotation technique for the identification of Cryptosporidium spp. oocysts, followed by PCR/sequencing analyses of the partial sequence of the 18S rDNA gene to determine the Cryptosporidium species. Of the 315 samples collected, only 10 (186/5.38%) from area A were positive for Cryptosporidium. The nucleotide sequence and phylogenetic analyses identified that all samples were identical (100%) and belonged to Cryptosporidium baileyi species, which is closely related to gastric species and of importance in animal health.
publisher Universidade Federal de Santa Maria
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000500501
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