Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog

Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog

ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The gestational age was estimated to be 23±1 day. Through qPCR, the presence of L. infantum DNA was detected in all the samples analyzed (n=7), including the embryo, conversely through cPCR, only four samples (vagina, cervix, uterine body and embryo) were positive. This study demonstrated that transmission of L. infantum from a pregnant dog to the embryo might occur in the early days of pregnancy. In conclusion, this is the first report showing L. infantum infecting a canine embryo.

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Main Authors: Oliveira,Vinícius Vasconcelos Gomes de, Ramos,Rafael Antonio Nascimento, Ramos,Carlos Alberto do Nascimento, Guerra,Neurisvan Ramos, Maia,Frederico Celso Lyra, Alves,Leucio Câmara, Silva Junior,Valdemiro Amaro da
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553
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spelling oai:scielo:S0103-847820170001005532016-11-21Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dogOliveira,Vinícius Vasconcelos Gomes deRamos,Rafael Antonio NascimentoRamos,Carlos Alberto do NascimentoGuerra,Neurisvan RamosMaia,Frederico Celso LyraAlves,Leucio CâmaraSilva Junior,Valdemiro Amaro da leishmaniasis dog vertical infection embryo ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The gestational age was estimated to be 23±1 day. Through qPCR, the presence of L. infantum DNA was detected in all the samples analyzed (n=7), including the embryo, conversely through cPCR, only four samples (vagina, cervix, uterine body and embryo) were positive. This study demonstrated that transmission of L. infantum from a pregnant dog to the embryo might occur in the early days of pregnancy. In conclusion, this is the first report showing L. infantum infecting a canine embryo.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.47 n.1 20172017-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553en10.1590/0103-8478cr20160553
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Oliveira,Vinícius Vasconcelos Gomes de
Ramos,Rafael Antonio Nascimento
Ramos,Carlos Alberto do Nascimento
Guerra,Neurisvan Ramos
Maia,Frederico Celso Lyra
Alves,Leucio Câmara
Silva Junior,Valdemiro Amaro da
spellingShingle Oliveira,Vinícius Vasconcelos Gomes de
Ramos,Rafael Antonio Nascimento
Ramos,Carlos Alberto do Nascimento
Guerra,Neurisvan Ramos
Maia,Frederico Celso Lyra
Alves,Leucio Câmara
Silva Junior,Valdemiro Amaro da
Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
author_facet Oliveira,Vinícius Vasconcelos Gomes de
Ramos,Rafael Antonio Nascimento
Ramos,Carlos Alberto do Nascimento
Guerra,Neurisvan Ramos
Maia,Frederico Celso Lyra
Alves,Leucio Câmara
Silva Junior,Valdemiro Amaro da
author_sort Oliveira,Vinícius Vasconcelos Gomes de
title Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
title_short Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
title_full Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
title_fullStr Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
title_full_unstemmed Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
title_sort molecular evidence of early vertical transmission of leishmania ( leishmania ) infantum in a dog
description ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The gestational age was estimated to be 23±1 day. Through qPCR, the presence of L. infantum DNA was detected in all the samples analyzed (n=7), including the embryo, conversely through cPCR, only four samples (vagina, cervix, uterine body and embryo) were positive. This study demonstrated that transmission of L. infantum from a pregnant dog to the embryo might occur in the early days of pregnancy. In conclusion, this is the first report showing L. infantum infecting a canine embryo.
publisher Universidade Federal de Santa Maria
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553
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