Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)

Almost 90% of species of the genus Passiflora are native to the American continent, with high commercial value due to the fact that some species are used for human food while others have ornamental and medical qualities. Passiflora serrato-digitata is one of the species that integrates the Paraná Agronomic Institute germoplasm bank at its experimental base in Londrina, PR, Brazil. Collected flower buds were fixed in ethanol/acetic acid (3:1 v/v) for 24h, transferred to 70% alcohol and stored under refrigeration. Slides were prepared by the squashing technique and stained with 1.0% propionic carmine; they were analyzed under an optic microscope. Irregularities in the chromosome segregation process of P. serrato-digitata have been verified by meiotic behavior analysis. These comprised precocious migration to poles in metaphase I and II, non-oriented chromosomes in metaphase plate in metaphase I and II, laggard chromosomes in anaphase I and II towards the formation of micronucleus in telophase I and II, and microspores in tetrads. Chromosome stickiness was another irregularity reported in the Passiflora genus for the first time. These irregularities which also contributed to the formation of monads, dyads and triads, resulted in normal imbalanced 2n and 4n microspores. According to the observed Meiotic Index of 71.83%, this species is not meiotically stable.

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Main Authors: Kiihl,Paulo Roberto Peres, Pereira,Andréia Rodrigues Alonso, Godoy,Sara Mataroli de, Stenzel,Neusa Maria Colauto, Risso-Pascotto,Claudicéia
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000600016
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spelling oai:scielo:S0103-847820110006000162011-07-18Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)Kiihl,Paulo Roberto PeresPereira,Andréia Rodrigues AlonsoGodoy,Sara Mataroli deStenzel,Neusa Maria ColautoRisso-Pascotto,Claudicéia microsporogenesis meiosis 2n gametes Almost 90% of species of the genus Passiflora are native to the American continent, with high commercial value due to the fact that some species are used for human food while others have ornamental and medical qualities. Passiflora serrato-digitata is one of the species that integrates the Paraná Agronomic Institute germoplasm bank at its experimental base in Londrina, PR, Brazil. Collected flower buds were fixed in ethanol/acetic acid (3:1 v/v) for 24h, transferred to 70% alcohol and stored under refrigeration. Slides were prepared by the squashing technique and stained with 1.0% propionic carmine; they were analyzed under an optic microscope. Irregularities in the chromosome segregation process of P. serrato-digitata have been verified by meiotic behavior analysis. These comprised precocious migration to poles in metaphase I and II, non-oriented chromosomes in metaphase plate in metaphase I and II, laggard chromosomes in anaphase I and II towards the formation of micronucleus in telophase I and II, and microspores in tetrads. Chromosome stickiness was another irregularity reported in the Passiflora genus for the first time. These irregularities which also contributed to the formation of monads, dyads and triads, resulted in normal imbalanced 2n and 4n microspores. According to the observed Meiotic Index of 71.83%, this species is not meiotically stable.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.41 n.6 20112011-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000600016en10.1590/S0103-84782011005000076
institution SCIELO
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Kiihl,Paulo Roberto Peres
Pereira,Andréia Rodrigues Alonso
Godoy,Sara Mataroli de
Stenzel,Neusa Maria Colauto
Risso-Pascotto,Claudicéia
spellingShingle Kiihl,Paulo Roberto Peres
Pereira,Andréia Rodrigues Alonso
Godoy,Sara Mataroli de
Stenzel,Neusa Maria Colauto
Risso-Pascotto,Claudicéia
Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)
author_facet Kiihl,Paulo Roberto Peres
Pereira,Andréia Rodrigues Alonso
Godoy,Sara Mataroli de
Stenzel,Neusa Maria Colauto
Risso-Pascotto,Claudicéia
author_sort Kiihl,Paulo Roberto Peres
title Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)
title_short Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)
title_full Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)
title_fullStr Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)
title_full_unstemmed Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)
title_sort chromosome stickiness during meiotic behavior analysis of passiflora serrato-digitata l. (passifloraceae)
description Almost 90% of species of the genus Passiflora are native to the American continent, with high commercial value due to the fact that some species are used for human food while others have ornamental and medical qualities. Passiflora serrato-digitata is one of the species that integrates the Paraná Agronomic Institute germoplasm bank at its experimental base in Londrina, PR, Brazil. Collected flower buds were fixed in ethanol/acetic acid (3:1 v/v) for 24h, transferred to 70% alcohol and stored under refrigeration. Slides were prepared by the squashing technique and stained with 1.0% propionic carmine; they were analyzed under an optic microscope. Irregularities in the chromosome segregation process of P. serrato-digitata have been verified by meiotic behavior analysis. These comprised precocious migration to poles in metaphase I and II, non-oriented chromosomes in metaphase plate in metaphase I and II, laggard chromosomes in anaphase I and II towards the formation of micronucleus in telophase I and II, and microspores in tetrads. Chromosome stickiness was another irregularity reported in the Passiflora genus for the first time. These irregularities which also contributed to the formation of monads, dyads and triads, resulted in normal imbalanced 2n and 4n microspores. According to the observed Meiotic Index of 71.83%, this species is not meiotically stable.
publisher Universidade Federal de Santa Maria
publishDate 2011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000600016
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