INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES

To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did not affect (P<0.05) nuclear maturation. However, changing the supplement altered the cytoplasmic maturation, with higher rate (P<0.05) observed in the l-pFF group. To determine the effect of the presence of hormone and/or supplement during maturation, the oocytes were cultured either in presence of TCM-199 alone, with hormones, with 10% l-pFF or with hormones and 10% l-pFF. The highest proportion of oocytes undergoing nuclear and cytoplasmic maturation was obtained when both hormones and follicular fluid were present. Cumulus expansion had a significant (P<0.05) effect on cytoplasmic maturation with the non-expanded groups showing a lower percentage of maturation in all groups. When the adequacy of gonadotropins levels were evaluated by adding higher or lower concentrations into the maturation medium neither beneficial nor detrimental effects were observed in either nuclear or cytoplasmic maturation. These results suggest that changes in the composition of the medium can alter the percentage of oocytes completing maturation. Follicular fluid combined with hormones was found to give better conditions for pig oocyte maturation in vitro.

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Main Authors: Dode,Margot Alves Nunes, Graves,Charles
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2001
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100016
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spelling oai:scielo:S0103-847820010001000162005-06-10INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTESDode,Margot Alves NunesGraves,Charles follicular fluid oocyte maturation gonadotrophins porcine To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did not affect (P<0.05) nuclear maturation. However, changing the supplement altered the cytoplasmic maturation, with higher rate (P<0.05) observed in the l-pFF group. To determine the effect of the presence of hormone and/or supplement during maturation, the oocytes were cultured either in presence of TCM-199 alone, with hormones, with 10% l-pFF or with hormones and 10% l-pFF. The highest proportion of oocytes undergoing nuclear and cytoplasmic maturation was obtained when both hormones and follicular fluid were present. Cumulus expansion had a significant (P<0.05) effect on cytoplasmic maturation with the non-expanded groups showing a lower percentage of maturation in all groups. When the adequacy of gonadotropins levels were evaluated by adding higher or lower concentrations into the maturation medium neither beneficial nor detrimental effects were observed in either nuclear or cytoplasmic maturation. These results suggest that changes in the composition of the medium can alter the percentage of oocytes completing maturation. Follicular fluid combined with hormones was found to give better conditions for pig oocyte maturation in vitro.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.31 n.1 20012001-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100016en10.1590/S0103-84782001000100016
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Dode,Margot Alves Nunes
Graves,Charles
spellingShingle Dode,Margot Alves Nunes
Graves,Charles
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES
author_facet Dode,Margot Alves Nunes
Graves,Charles
author_sort Dode,Margot Alves Nunes
title INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES
title_short INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES
title_full INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES
title_fullStr INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES
title_full_unstemmed INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES
title_sort influence of hormones and follicular fluid on maturation of pig oocytes
description To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did not affect (P<0.05) nuclear maturation. However, changing the supplement altered the cytoplasmic maturation, with higher rate (P<0.05) observed in the l-pFF group. To determine the effect of the presence of hormone and/or supplement during maturation, the oocytes were cultured either in presence of TCM-199 alone, with hormones, with 10% l-pFF or with hormones and 10% l-pFF. The highest proportion of oocytes undergoing nuclear and cytoplasmic maturation was obtained when both hormones and follicular fluid were present. Cumulus expansion had a significant (P<0.05) effect on cytoplasmic maturation with the non-expanded groups showing a lower percentage of maturation in all groups. When the adequacy of gonadotropins levels were evaluated by adding higher or lower concentrations into the maturation medium neither beneficial nor detrimental effects were observed in either nuclear or cytoplasmic maturation. These results suggest that changes in the composition of the medium can alter the percentage of oocytes completing maturation. Follicular fluid combined with hormones was found to give better conditions for pig oocyte maturation in vitro.
publisher Universidade Federal de Santa Maria
publishDate 2001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100016
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