Ultrasound device as a minimally invasive approach for caries dentin removal

Abstract The aim of this study was to evaluate the efficacy of an ultrasound device and the dentin surface morphology after removal of the caries dentin lesions by removal rate and scanning electron microscopy (SEM). The Knoop hardness test on the bovine dentin blocks (n = 20, 4x4x2mm) was performed to standardize the samples and only those with 38 ± 2 KHN were included. The dentin blocks were submitted to induction of artificial caries lesions, using the bacterial model. Strains of Streptococcus mutans and Lactobacillus acidophilus were used for 7 days. The caries dentin lesion was removed for 1 min, according to two methods: G1 - carbide bur under low-speed rotation (control group) and G2 - ultrasound device under refrigeration. For the removal rate, the samples were weighed 3 times: T0 (before induction), T1 (after induction) and T2 (after removal). Morphology evaluation of the residual dentin surface was performed by SEM. Data normality was verified by Shapiro-Wilk test (p ≥ 0.240). T-test for independent samples was applied to evaluate the removal rate. A significance level of 5% was adopted. G2 provided lower removal rate than G1 (G1: 3.68 mg and G2 = 2.26 mg). SEM images showed different morphological characteristics between the groups. G2 showed absent of smear layer, while G1 showed a visible smear layer over the surface. We concluded that ultrasound device provides minimally invasive removal with residual dentin exhibiting open dentin tubules and no smear layer formation and no bacteria, which infer the removal of the infected tissue.

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Main Authors: Besegato,João Felipe, Melo,Priscila Borges Gobbo de, Bernardi,Adilson César de Abreu, Bagnato,Vanderlei Salvador, Rastelli,Alessandra Nara de Souza
Format: Digital revista
Language:English
Published: Fundação Odontológica de Ribeirão Preto 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000100057
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spelling oai:scielo:S0103-644020220001000572022-02-25Ultrasound device as a minimally invasive approach for caries dentin removalBesegato,João FelipeMelo,Priscila Borges Gobbo deBernardi,Adilson César de AbreuBagnato,Vanderlei SalvadorRastelli,Alessandra Nara de Souza Dental caries ultrasound energy caries removal scanning electron microscopy minimally invasive dentistry Abstract The aim of this study was to evaluate the efficacy of an ultrasound device and the dentin surface morphology after removal of the caries dentin lesions by removal rate and scanning electron microscopy (SEM). The Knoop hardness test on the bovine dentin blocks (n = 20, 4x4x2mm) was performed to standardize the samples and only those with 38 ± 2 KHN were included. The dentin blocks were submitted to induction of artificial caries lesions, using the bacterial model. Strains of Streptococcus mutans and Lactobacillus acidophilus were used for 7 days. The caries dentin lesion was removed for 1 min, according to two methods: G1 - carbide bur under low-speed rotation (control group) and G2 - ultrasound device under refrigeration. For the removal rate, the samples were weighed 3 times: T0 (before induction), T1 (after induction) and T2 (after removal). Morphology evaluation of the residual dentin surface was performed by SEM. Data normality was verified by Shapiro-Wilk test (p ≥ 0.240). T-test for independent samples was applied to evaluate the removal rate. A significance level of 5% was adopted. G2 provided lower removal rate than G1 (G1: 3.68 mg and G2 = 2.26 mg). SEM images showed different morphological characteristics between the groups. G2 showed absent of smear layer, while G1 showed a visible smear layer over the surface. We concluded that ultrasound device provides minimally invasive removal with residual dentin exhibiting open dentin tubules and no smear layer formation and no bacteria, which infer the removal of the infected tissue.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.33 n.1 20222022-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000100057en10.1590/0103-6440202203878
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Besegato,João Felipe
Melo,Priscila Borges Gobbo de
Bernardi,Adilson César de Abreu
Bagnato,Vanderlei Salvador
Rastelli,Alessandra Nara de Souza
spellingShingle Besegato,João Felipe
Melo,Priscila Borges Gobbo de
Bernardi,Adilson César de Abreu
Bagnato,Vanderlei Salvador
Rastelli,Alessandra Nara de Souza
Ultrasound device as a minimally invasive approach for caries dentin removal
author_facet Besegato,João Felipe
Melo,Priscila Borges Gobbo de
Bernardi,Adilson César de Abreu
Bagnato,Vanderlei Salvador
Rastelli,Alessandra Nara de Souza
author_sort Besegato,João Felipe
title Ultrasound device as a minimally invasive approach for caries dentin removal
title_short Ultrasound device as a minimally invasive approach for caries dentin removal
title_full Ultrasound device as a minimally invasive approach for caries dentin removal
title_fullStr Ultrasound device as a minimally invasive approach for caries dentin removal
title_full_unstemmed Ultrasound device as a minimally invasive approach for caries dentin removal
title_sort ultrasound device as a minimally invasive approach for caries dentin removal
description Abstract The aim of this study was to evaluate the efficacy of an ultrasound device and the dentin surface morphology after removal of the caries dentin lesions by removal rate and scanning electron microscopy (SEM). The Knoop hardness test on the bovine dentin blocks (n = 20, 4x4x2mm) was performed to standardize the samples and only those with 38 ± 2 KHN were included. The dentin blocks were submitted to induction of artificial caries lesions, using the bacterial model. Strains of Streptococcus mutans and Lactobacillus acidophilus were used for 7 days. The caries dentin lesion was removed for 1 min, according to two methods: G1 - carbide bur under low-speed rotation (control group) and G2 - ultrasound device under refrigeration. For the removal rate, the samples were weighed 3 times: T0 (before induction), T1 (after induction) and T2 (after removal). Morphology evaluation of the residual dentin surface was performed by SEM. Data normality was verified by Shapiro-Wilk test (p ≥ 0.240). T-test for independent samples was applied to evaluate the removal rate. A significance level of 5% was adopted. G2 provided lower removal rate than G1 (G1: 3.68 mg and G2 = 2.26 mg). SEM images showed different morphological characteristics between the groups. G2 showed absent of smear layer, while G1 showed a visible smear layer over the surface. We concluded that ultrasound device provides minimally invasive removal with residual dentin exhibiting open dentin tubules and no smear layer formation and no bacteria, which infer the removal of the infected tissue.
publisher Fundação Odontológica de Ribeirão Preto
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000100057
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