Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study
Abstract This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to describe the biological functions of proteins identified in pulp tissue. Samples were obtained from six patients treated at the Araçatuba School of Dentistry and were divided into three groups: normal pulp - from teeth extracted for orthodontic indication; inflamed pulp and necrotic pulp - from patients diagnosed with irreversible pulpitis and chronic apical periodontitis, respectively. After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the groups was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm. A total of 465 human proteins were identified in all groups. The most expressed proteins in the inflamed pulp group in relation to the normal pulp group were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins. Expression levels of albumins, immunoglobulins and alpha-2-macroglobulin were higher in the necrotic pulp group than in the inflamed pulp group. As for the qualitative analysis, the most prevalent protein functions in the normal pulp group were metabolic and energetic pathways; in the inflamed pulp group: cellular communication and signal transduction; and regulation and repair of DNA/RNA, while in the necrotic pulp group proteins were associated with the immune response. Thus, proteomic analysis showed quantitative and qualitative differences in protein expression in different types of pulp conditions.
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Fundação Odontológica de Ribeirão Preto
2020
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oai:scielo:S0103-644020200003003192020-07-09Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot StudyLoureiro,CarolineBuzalaf,Marília Afonso RabeloPessan,Juliano PelimMoraes,Felipe Ricardo Nunes dePelá,Vinícius TaioquiVentura,Talita Mendes OliveiraJacinto,Rogério de Castilho proteomic analysis dental pulp pulpitis dental pulp necrosis endodontics Abstract This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to describe the biological functions of proteins identified in pulp tissue. Samples were obtained from six patients treated at the Araçatuba School of Dentistry and were divided into three groups: normal pulp - from teeth extracted for orthodontic indication; inflamed pulp and necrotic pulp - from patients diagnosed with irreversible pulpitis and chronic apical periodontitis, respectively. After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the groups was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm. A total of 465 human proteins were identified in all groups. The most expressed proteins in the inflamed pulp group in relation to the normal pulp group were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins. Expression levels of albumins, immunoglobulins and alpha-2-macroglobulin were higher in the necrotic pulp group than in the inflamed pulp group. As for the qualitative analysis, the most prevalent protein functions in the normal pulp group were metabolic and energetic pathways; in the inflamed pulp group: cellular communication and signal transduction; and regulation and repair of DNA/RNA, while in the necrotic pulp group proteins were associated with the immune response. Thus, proteomic analysis showed quantitative and qualitative differences in protein expression in different types of pulp conditions.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.31 n.3 20202020-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000300319en10.1590/0103-6440202003167 |
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Loureiro,Caroline Buzalaf,Marília Afonso Rabelo Pessan,Juliano Pelim Moraes,Felipe Ricardo Nunes de Pelá,Vinícius Taioqui Ventura,Talita Mendes Oliveira Jacinto,Rogério de Castilho |
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Loureiro,Caroline Buzalaf,Marília Afonso Rabelo Pessan,Juliano Pelim Moraes,Felipe Ricardo Nunes de Pelá,Vinícius Taioqui Ventura,Talita Mendes Oliveira Jacinto,Rogério de Castilho Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
author_facet |
Loureiro,Caroline Buzalaf,Marília Afonso Rabelo Pessan,Juliano Pelim Moraes,Felipe Ricardo Nunes de Pelá,Vinícius Taioqui Ventura,Talita Mendes Oliveira Jacinto,Rogério de Castilho |
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Loureiro,Caroline |
title |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_short |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_full |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_fullStr |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_full_unstemmed |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_sort |
comparative analysis of the proteomic profile of the dental pulp in different conditions. a pilot study |
description |
Abstract This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to describe the biological functions of proteins identified in pulp tissue. Samples were obtained from six patients treated at the Araçatuba School of Dentistry and were divided into three groups: normal pulp - from teeth extracted for orthodontic indication; inflamed pulp and necrotic pulp - from patients diagnosed with irreversible pulpitis and chronic apical periodontitis, respectively. After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the groups was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm. A total of 465 human proteins were identified in all groups. The most expressed proteins in the inflamed pulp group in relation to the normal pulp group were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins. Expression levels of albumins, immunoglobulins and alpha-2-macroglobulin were higher in the necrotic pulp group than in the inflamed pulp group. As for the qualitative analysis, the most prevalent protein functions in the normal pulp group were metabolic and energetic pathways; in the inflamed pulp group: cellular communication and signal transduction; and regulation and repair of DNA/RNA, while in the necrotic pulp group proteins were associated with the immune response. Thus, proteomic analysis showed quantitative and qualitative differences in protein expression in different types of pulp conditions. |
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Fundação Odontológica de Ribeirão Preto |
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2020 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000300319 |
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