Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
Abstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.
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Fundação Odontológica de Ribeirão Preto
2020
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oai:scielo:S0103-644020200001000192020-03-02Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical LesionsSilva-Sousa,Alice CorrêaMazzi-Chaves,Jardel FranciscoFreitas,Jessica VavassoriSalles,Alessandro GuimarãesSegato,Raquel Assed Bezerra da SilvaSilva,Léa Assed Bezerra daAntunes,Lívia Azeredo AlvesAntunes,Leonardo SantosBaratto-Filho,FlaresSousa-Neto,Manoel DamiãoKüchler,Erika Calvano persistent apical periodontitis genetic polymorphisms vitamin d estrogen Abstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.31 n.1 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100019en10.1590/0103-644020200 |
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Silva-Sousa,Alice Corrêa Mazzi-Chaves,Jardel Francisco Freitas,Jessica Vavassori Salles,Alessandro Guimarães Segato,Raquel Assed Bezerra da Silva Silva,Léa Assed Bezerra da Antunes,Lívia Azeredo Alves Antunes,Leonardo Santos Baratto-Filho,Flares Sousa-Neto,Manoel Damião Küchler,Erika Calvano |
| spellingShingle |
Silva-Sousa,Alice Corrêa Mazzi-Chaves,Jardel Francisco Freitas,Jessica Vavassori Salles,Alessandro Guimarães Segato,Raquel Assed Bezerra da Silva Silva,Léa Assed Bezerra da Antunes,Lívia Azeredo Alves Antunes,Leonardo Santos Baratto-Filho,Flares Sousa-Neto,Manoel Damião Küchler,Erika Calvano Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions |
| author_facet |
Silva-Sousa,Alice Corrêa Mazzi-Chaves,Jardel Francisco Freitas,Jessica Vavassori Salles,Alessandro Guimarães Segato,Raquel Assed Bezerra da Silva Silva,Léa Assed Bezerra da Antunes,Lívia Azeredo Alves Antunes,Leonardo Santos Baratto-Filho,Flares Sousa-Neto,Manoel Damião Küchler,Erika Calvano |
| author_sort |
Silva-Sousa,Alice Corrêa |
| title |
Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions |
| title_short |
Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions |
| title_full |
Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions |
| title_fullStr |
Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions |
| title_full_unstemmed |
Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions |
| title_sort |
association between estrogen, vitamin d and microrna17 gene polymorphisms and periapical lesions |
| description |
Abstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP. |
| publisher |
Fundação Odontológica de Ribeirão Preto |
| publishDate |
2020 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100019 |
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