Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis

Abstract This study correlated the bond strength (BS) and dentin penetration of different sealers by push-out test and Confocal Laser Scanning Microscopy (CLSM) analysis. Forty-five root canals were prepared according to the crown-down technique and filled with gutta-percha associated to the following sealers (n=15): Endofill, AH Plus and MTA Fillapex. Five canals of each group were filled with the sealers added with 0.1% Rhodamine B dye. Next, the specimens were transversely sectioned and submitted to the push-out test (n=10) and CLSM analysis (n=5). The BS data showed the following means (MPa) and standard deviation: AH Plus (4.17±1.86); MTA Fillapex (3.13±1.96) and Endofill (2.10±1.03). Statistical analysis (two-way ANOVA, α=0.05) showed significant difference among sealers (p<0.001) and root canal thirds (p<0.001). The BS results of Endofill and MTA Fillapex were statistically similar (p>0.05), however, they were statistically different from AH Plus (p<0.001). The regional analysis of BS showed similarity between middle and apical thirds (p>0.05), and both were different from coronal portion (p<0.001). CLSM analysis verified tags formation in all groups and higher penetration of the specimens filled with AH Plus (p<0.05). The Kendall test (correlation between BS to dentin and sealer penetration into dentinal tubules) and the Pearson test (between failures pattern and sealer penetration into dentinal tubules) did not show correlation between the variables evaluated for all the tested sealers (p>0.05). AH Plus group had higher BS to dentin, and deeper tags formation than the other sealers. There was no significant correlation between BS and intratubular penetration of the tested sealers.

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Main Authors: Tedesco,Maybell, Chain,Marcelo Carvalho, Felippe,Wilson Tadeu, Alves,Ana Maria Hecke, Garcia,Lucas da Fonseca Roberti, Bortoluzzi,Eduardo Antunes, Cordeiro,Mabel Rodriguez, Teixeira,Cleonice Silveira
Format: Digital revista
Language:English
Published: Fundação Odontológica de Ribeirão Preto 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000600555
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spelling oai:scielo:S0103-644020190006005552019-11-25Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM AnalysisTedesco,MaybellChain,Marcelo CarvalhoFelippe,Wilson TadeuAlves,Ana Maria HeckeGarcia,Lucas da Fonseca RobertiBortoluzzi,Eduardo AntunesCordeiro,Mabel RodriguezTeixeira,Cleonice Silveira endodontics root canal obturation root canal filling materials confocal Abstract This study correlated the bond strength (BS) and dentin penetration of different sealers by push-out test and Confocal Laser Scanning Microscopy (CLSM) analysis. Forty-five root canals were prepared according to the crown-down technique and filled with gutta-percha associated to the following sealers (n=15): Endofill, AH Plus and MTA Fillapex. Five canals of each group were filled with the sealers added with 0.1% Rhodamine B dye. Next, the specimens were transversely sectioned and submitted to the push-out test (n=10) and CLSM analysis (n=5). The BS data showed the following means (MPa) and standard deviation: AH Plus (4.17±1.86); MTA Fillapex (3.13±1.96) and Endofill (2.10±1.03). Statistical analysis (two-way ANOVA, α=0.05) showed significant difference among sealers (p<0.001) and root canal thirds (p<0.001). The BS results of Endofill and MTA Fillapex were statistically similar (p>0.05), however, they were statistically different from AH Plus (p<0.001). The regional analysis of BS showed similarity between middle and apical thirds (p>0.05), and both were different from coronal portion (p<0.001). CLSM analysis verified tags formation in all groups and higher penetration of the specimens filled with AH Plus (p<0.05). The Kendall test (correlation between BS to dentin and sealer penetration into dentinal tubules) and the Pearson test (between failures pattern and sealer penetration into dentinal tubules) did not show correlation between the variables evaluated for all the tested sealers (p>0.05). AH Plus group had higher BS to dentin, and deeper tags formation than the other sealers. There was no significant correlation between BS and intratubular penetration of the tested sealers.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.30 n.6 20192019-11-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000600555en10.1590/0103-6440201902766
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country Brasil
countrycode BR
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Tedesco,Maybell
Chain,Marcelo Carvalho
Felippe,Wilson Tadeu
Alves,Ana Maria Hecke
Garcia,Lucas da Fonseca Roberti
Bortoluzzi,Eduardo Antunes
Cordeiro,Mabel Rodriguez
Teixeira,Cleonice Silveira
spellingShingle Tedesco,Maybell
Chain,Marcelo Carvalho
Felippe,Wilson Tadeu
Alves,Ana Maria Hecke
Garcia,Lucas da Fonseca Roberti
Bortoluzzi,Eduardo Antunes
Cordeiro,Mabel Rodriguez
Teixeira,Cleonice Silveira
Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis
author_facet Tedesco,Maybell
Chain,Marcelo Carvalho
Felippe,Wilson Tadeu
Alves,Ana Maria Hecke
Garcia,Lucas da Fonseca Roberti
Bortoluzzi,Eduardo Antunes
Cordeiro,Mabel Rodriguez
Teixeira,Cleonice Silveira
author_sort Tedesco,Maybell
title Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis
title_short Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis
title_full Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis
title_fullStr Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis
title_full_unstemmed Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis
title_sort correlation between bond strength to dentin and sealers penetration by push-out test and clsm analysis
description Abstract This study correlated the bond strength (BS) and dentin penetration of different sealers by push-out test and Confocal Laser Scanning Microscopy (CLSM) analysis. Forty-five root canals were prepared according to the crown-down technique and filled with gutta-percha associated to the following sealers (n=15): Endofill, AH Plus and MTA Fillapex. Five canals of each group were filled with the sealers added with 0.1% Rhodamine B dye. Next, the specimens were transversely sectioned and submitted to the push-out test (n=10) and CLSM analysis (n=5). The BS data showed the following means (MPa) and standard deviation: AH Plus (4.17±1.86); MTA Fillapex (3.13±1.96) and Endofill (2.10±1.03). Statistical analysis (two-way ANOVA, α=0.05) showed significant difference among sealers (p<0.001) and root canal thirds (p<0.001). The BS results of Endofill and MTA Fillapex were statistically similar (p>0.05), however, they were statistically different from AH Plus (p<0.001). The regional analysis of BS showed similarity between middle and apical thirds (p>0.05), and both were different from coronal portion (p<0.001). CLSM analysis verified tags formation in all groups and higher penetration of the specimens filled with AH Plus (p<0.05). The Kendall test (correlation between BS to dentin and sealer penetration into dentinal tubules) and the Pearson test (between failures pattern and sealer penetration into dentinal tubules) did not show correlation between the variables evaluated for all the tested sealers (p>0.05). AH Plus group had higher BS to dentin, and deeper tags formation than the other sealers. There was no significant correlation between BS and intratubular penetration of the tested sealers.
publisher Fundação Odontológica de Ribeirão Preto
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000600555
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