Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material

Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material

Abstract New mineral trioxide aggregate (MTA) formulations are constantly introduced in the market, usually in a powder-and-liquid form. Bioceramic (Bio-C) Repair is a ready-for-use material suggested as substitute for MTA, but its properties need to be studied. This study evaluated the cytotoxicity, biocompatibility and biomineralization of Bio-C Repair compared to MTA Repair High-Plasticity (MTA-HP) and white MTA-Angelus (MTA-Ang). L929 fibroblasts were exposed to material-extracted (undiluted, ½ and ¼ dilutions; 6, 24 and 48h). Polyethylene tubes with material or empty (control) were implanted in the subcutaneous tissue of rats. After 7 and 30 days (n=8), the specimens were removed for analysis (hematoxylin-eosin, von Kossa and polarized light). Cytotoxicity data were statistically analyzed by two-way ANOVA, and biocompatibility data by Kruskal-Wallis and Dunn tests (p<0.05). The cells exposed to the materials had greater viability at most of the periods compared with control (p<0.05). The undiluted and ½ dilutions of MTA-HP extract showed higher cytocompatibility than Bio-C Repair at 6 h and with the ¼ dilution at 24 h (p<0.05); the white MTA-Ang showed higher cytocompatibility than Bio-C Repair at most of periods (p<0.05). The undiluted white MTA-Ang extract had higher cytocompatibility at 6 and 24h than MTA-HP, and with ½ dilution at 24h (p<0.05). The materials’ cytocompatibility was similar at 48h for most dilutions (p>0.05). At 7 and 30 days, the groups had moderate and mild inflammation, respectively (p>0.05). All materials showed positive structures for von Kossa and polarized light. In conclusion, Bio-C Repair had similar cytocompatibility to MTA-based materials is biocompatible and induces biomineralization.

Saved in:
Bibliographic Details
Main Authors: Benetti,Francine, Queiroz,Índia Olinta de Azevedo, Cosme-Silva,Leopoldo, Conti,Leticia Citelli, Oliveira,Sandra Helena Penha de, Cintra,Luciano Tavares Angelo
Format: Digital revista
Language:English
Published: Fundação Odontológica de Ribeirão Preto 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000400325
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0103-64402019000400325
record_format ojs
spelling oai:scielo:S0103-644020190004003252019-08-01Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair MaterialBenetti,FrancineQueiroz,Índia Olinta de AzevedoCosme-Silva,LeopoldoConti,Leticia CitelliOliveira,Sandra Helena Penha deCintra,Luciano Tavares Angelo biocompatible materials cytotoxicity dental materials endodontics materials testing Abstract New mineral trioxide aggregate (MTA) formulations are constantly introduced in the market, usually in a powder-and-liquid form. Bioceramic (Bio-C) Repair is a ready-for-use material suggested as substitute for MTA, but its properties need to be studied. This study evaluated the cytotoxicity, biocompatibility and biomineralization of Bio-C Repair compared to MTA Repair High-Plasticity (MTA-HP) and white MTA-Angelus (MTA-Ang). L929 fibroblasts were exposed to material-extracted (undiluted, ½ and ¼ dilutions; 6, 24 and 48h). Polyethylene tubes with material or empty (control) were implanted in the subcutaneous tissue of rats. After 7 and 30 days (n=8), the specimens were removed for analysis (hematoxylin-eosin, von Kossa and polarized light). Cytotoxicity data were statistically analyzed by two-way ANOVA, and biocompatibility data by Kruskal-Wallis and Dunn tests (p<0.05). The cells exposed to the materials had greater viability at most of the periods compared with control (p<0.05). The undiluted and ½ dilutions of MTA-HP extract showed higher cytocompatibility than Bio-C Repair at 6 h and with the ¼ dilution at 24 h (p<0.05); the white MTA-Ang showed higher cytocompatibility than Bio-C Repair at most of periods (p<0.05). The undiluted white MTA-Ang extract had higher cytocompatibility at 6 and 24h than MTA-HP, and with ½ dilution at 24h (p<0.05). The materials’ cytocompatibility was similar at 48h for most dilutions (p>0.05). At 7 and 30 days, the groups had moderate and mild inflammation, respectively (p>0.05). All materials showed positive structures for von Kossa and polarized light. In conclusion, Bio-C Repair had similar cytocompatibility to MTA-based materials is biocompatible and induces biomineralization.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.30 n.4 20192019-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000400325en10.1590/0103-6440201902457
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Benetti,Francine
Queiroz,Índia Olinta de Azevedo
Cosme-Silva,Leopoldo
Conti,Leticia Citelli
Oliveira,Sandra Helena Penha de
Cintra,Luciano Tavares Angelo
spellingShingle Benetti,Francine
Queiroz,Índia Olinta de Azevedo
Cosme-Silva,Leopoldo
Conti,Leticia Citelli
Oliveira,Sandra Helena Penha de
Cintra,Luciano Tavares Angelo
Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material
author_facet Benetti,Francine
Queiroz,Índia Olinta de Azevedo
Cosme-Silva,Leopoldo
Conti,Leticia Citelli
Oliveira,Sandra Helena Penha de
Cintra,Luciano Tavares Angelo
author_sort Benetti,Francine
title Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material
title_short Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material
title_full Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material
title_fullStr Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material
title_full_unstemmed Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material
title_sort cytotoxicity, biocompatibility and biomineralization of a new ready-for-use bioceramic repair material
description Abstract New mineral trioxide aggregate (MTA) formulations are constantly introduced in the market, usually in a powder-and-liquid form. Bioceramic (Bio-C) Repair is a ready-for-use material suggested as substitute for MTA, but its properties need to be studied. This study evaluated the cytotoxicity, biocompatibility and biomineralization of Bio-C Repair compared to MTA Repair High-Plasticity (MTA-HP) and white MTA-Angelus (MTA-Ang). L929 fibroblasts were exposed to material-extracted (undiluted, ½ and ¼ dilutions; 6, 24 and 48h). Polyethylene tubes with material or empty (control) were implanted in the subcutaneous tissue of rats. After 7 and 30 days (n=8), the specimens were removed for analysis (hematoxylin-eosin, von Kossa and polarized light). Cytotoxicity data were statistically analyzed by two-way ANOVA, and biocompatibility data by Kruskal-Wallis and Dunn tests (p<0.05). The cells exposed to the materials had greater viability at most of the periods compared with control (p<0.05). The undiluted and ½ dilutions of MTA-HP extract showed higher cytocompatibility than Bio-C Repair at 6 h and with the ¼ dilution at 24 h (p<0.05); the white MTA-Ang showed higher cytocompatibility than Bio-C Repair at most of periods (p<0.05). The undiluted white MTA-Ang extract had higher cytocompatibility at 6 and 24h than MTA-HP, and with ½ dilution at 24h (p<0.05). The materials’ cytocompatibility was similar at 48h for most dilutions (p>0.05). At 7 and 30 days, the groups had moderate and mild inflammation, respectively (p>0.05). All materials showed positive structures for von Kossa and polarized light. In conclusion, Bio-C Repair had similar cytocompatibility to MTA-based materials is biocompatible and induces biomineralization.
publisher Fundação Odontológica de Ribeirão Preto
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000400325
work_keys_str_mv AT benettifrancine cytotoxicitybiocompatibilityandbiomineralizationofanewreadyforusebioceramicrepairmaterial
AT queirozindiaolintadeazevedo cytotoxicitybiocompatibilityandbiomineralizationofanewreadyforusebioceramicrepairmaterial
AT cosmesilvaleopoldo cytotoxicitybiocompatibilityandbiomineralizationofanewreadyforusebioceramicrepairmaterial
AT contileticiacitelli cytotoxicitybiocompatibilityandbiomineralizationofanewreadyforusebioceramicrepairmaterial
AT oliveirasandrahelenapenhade cytotoxicitybiocompatibilityandbiomineralizationofanewreadyforusebioceramicrepairmaterial
AT cintralucianotavaresangelo cytotoxicitybiocompatibilityandbiomineralizationofanewreadyforusebioceramicrepairmaterial
_version_ 1756404620636192768

Similar Items