Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

Abstract The aim of this study was to evaluate markers of bone loss and immune response present in evolution of periodontal disease. One hundred and two Wistar rats were divided into three animals groups: PD0, without ligation and PD15 days and PD60 days, submitted to ligation placement with a sterile 3-0 silk cord in the cervical region of the upper first molar on both sides. Samples were obtained from the gingival tissue for histomorphometric analysis, immunohistochemical analysis of RANK, RANKL, OPG, characterization of the inflammatory infiltrate, quantification of nitric oxide, MCP-1, RANTES, IP10 chemokines, and expression of the TGF-b1, VEG, and bFGF. The number of inflammatory cells in gingival tissue was higher in PD60 samples. The collagen content and the area occupied by birefringent collagen fibers were lower for PD60. Differential leukocyte counting showed that there was a significantly higher polymorphonuclear influx in group PD15, while PD60 showed a greater number of lymphocytes. PD60 showed higher RANTES, IP-10, MCP-1 gene transcripts, as well as a higher nitric oxide concentration. Clinical evaluation revealed that the PD60 group presented an increase in furcal area. In conclusion, in this animal model the increase of RANK/RANKL and HGF markers is related to a specific immune response, and probably contributed to the evolution of periodontal disease. Investigating the effect of these biomarkers can help in targeted therapy for bone resorption, since blocking these can inhibit bone loss.

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Main Authors: Gibertoni,Fabrício, Sommer,Meire Ellen Ligia, Esquisatto,Marcelo Augusto Marretto, Amaral,Maria Esméria Corezola do, Oliveira,Camila Andrea de, Andrade,Thiago Antônio Moretti de, Mendonça,Fernanda Aparecida Sampaio, Santamaria-Jr,Milton, Felonato,Maíra
Format: Digital revista
Language:English
Published: Fundação Odontológica de Ribeirão Preto 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000600679
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spelling oai:scielo:S0103-644020170006006792017-11-30Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG SystemGibertoni,FabrícioSommer,Meire Ellen LigiaEsquisatto,Marcelo Augusto MarrettoAmaral,Maria Esméria Corezola doOliveira,Camila Andrea deAndrade,Thiago Antônio Moretti deMendonça,Fernanda Aparecida SampaioSantamaria-Jr,MiltonFelonato,Maíra periodontal disease cytokines chemokines leucocytes RANK/RANKL/OPG Abstract The aim of this study was to evaluate markers of bone loss and immune response present in evolution of periodontal disease. One hundred and two Wistar rats were divided into three animals groups: PD0, without ligation and PD15 days and PD60 days, submitted to ligation placement with a sterile 3-0 silk cord in the cervical region of the upper first molar on both sides. Samples were obtained from the gingival tissue for histomorphometric analysis, immunohistochemical analysis of RANK, RANKL, OPG, characterization of the inflammatory infiltrate, quantification of nitric oxide, MCP-1, RANTES, IP10 chemokines, and expression of the TGF-b1, VEG, and bFGF. The number of inflammatory cells in gingival tissue was higher in PD60 samples. The collagen content and the area occupied by birefringent collagen fibers were lower for PD60. Differential leukocyte counting showed that there was a significantly higher polymorphonuclear influx in group PD15, while PD60 showed a greater number of lymphocytes. PD60 showed higher RANTES, IP-10, MCP-1 gene transcripts, as well as a higher nitric oxide concentration. Clinical evaluation revealed that the PD60 group presented an increase in furcal area. In conclusion, in this animal model the increase of RANK/RANKL and HGF markers is related to a specific immune response, and probably contributed to the evolution of periodontal disease. Investigating the effect of these biomarkers can help in targeted therapy for bone resorption, since blocking these can inhibit bone loss.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.28 n.6 20172017-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000600679en10.1590/0103-6440201701407
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country Brasil
countrycode BR
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libraryname SciELO
language English
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author Gibertoni,Fabrício
Sommer,Meire Ellen Ligia
Esquisatto,Marcelo Augusto Marretto
Amaral,Maria Esméria Corezola do
Oliveira,Camila Andrea de
Andrade,Thiago Antônio Moretti de
Mendonça,Fernanda Aparecida Sampaio
Santamaria-Jr,Milton
Felonato,Maíra
spellingShingle Gibertoni,Fabrício
Sommer,Meire Ellen Ligia
Esquisatto,Marcelo Augusto Marretto
Amaral,Maria Esméria Corezola do
Oliveira,Camila Andrea de
Andrade,Thiago Antônio Moretti de
Mendonça,Fernanda Aparecida Sampaio
Santamaria-Jr,Milton
Felonato,Maíra
Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System
author_facet Gibertoni,Fabrício
Sommer,Meire Ellen Ligia
Esquisatto,Marcelo Augusto Marretto
Amaral,Maria Esméria Corezola do
Oliveira,Camila Andrea de
Andrade,Thiago Antônio Moretti de
Mendonça,Fernanda Aparecida Sampaio
Santamaria-Jr,Milton
Felonato,Maíra
author_sort Gibertoni,Fabrício
title Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System
title_short Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System
title_full Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System
title_fullStr Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System
title_full_unstemmed Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System
title_sort evolution of periodontal disease: immune response and rank/rankl/opg system
description Abstract The aim of this study was to evaluate markers of bone loss and immune response present in evolution of periodontal disease. One hundred and two Wistar rats were divided into three animals groups: PD0, without ligation and PD15 days and PD60 days, submitted to ligation placement with a sterile 3-0 silk cord in the cervical region of the upper first molar on both sides. Samples were obtained from the gingival tissue for histomorphometric analysis, immunohistochemical analysis of RANK, RANKL, OPG, characterization of the inflammatory infiltrate, quantification of nitric oxide, MCP-1, RANTES, IP10 chemokines, and expression of the TGF-b1, VEG, and bFGF. The number of inflammatory cells in gingival tissue was higher in PD60 samples. The collagen content and the area occupied by birefringent collagen fibers were lower for PD60. Differential leukocyte counting showed that there was a significantly higher polymorphonuclear influx in group PD15, while PD60 showed a greater number of lymphocytes. PD60 showed higher RANTES, IP-10, MCP-1 gene transcripts, as well as a higher nitric oxide concentration. Clinical evaluation revealed that the PD60 group presented an increase in furcal area. In conclusion, in this animal model the increase of RANK/RANKL and HGF markers is related to a specific immune response, and probably contributed to the evolution of periodontal disease. Investigating the effect of these biomarkers can help in targeted therapy for bone resorption, since blocking these can inhibit bone loss.
publisher Fundação Odontológica de Ribeirão Preto
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000600679
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