Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis

This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.

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Main Authors: Farac,Roberta Vieira, Pizzolitto,Antonio Carlos, Tanomaru,Juliane Maria Guerreiro, Morgental,Renata Dornelles, Lima,Regina Karla de Pontes, Bonetti-Filho,Idomeo
Format: Digital revista
Language:English
Published: Fundação Odontológica de Ribeirão Preto 2013
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000200103
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spelling oai:scielo:S0103-644020130002001032013-05-27Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalisFarac,Roberta VieiraPizzolitto,Antonio CarlosTanomaru,Juliane Maria GuerreiroMorgental,Renata DornellesLima,Regina Karla de PontesBonetti-Filho,Idomeo calcium hydroxide Endodontics Enterococcus faecalis ozone root canal preparation This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.24 n.2 20132013-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000200103en10.1590/0103-6440201301992
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Farac,Roberta Vieira
Pizzolitto,Antonio Carlos
Tanomaru,Juliane Maria Guerreiro
Morgental,Renata Dornelles
Lima,Regina Karla de Pontes
Bonetti-Filho,Idomeo
spellingShingle Farac,Roberta Vieira
Pizzolitto,Antonio Carlos
Tanomaru,Juliane Maria Guerreiro
Morgental,Renata Dornelles
Lima,Regina Karla de Pontes
Bonetti-Filho,Idomeo
Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis
author_facet Farac,Roberta Vieira
Pizzolitto,Antonio Carlos
Tanomaru,Juliane Maria Guerreiro
Morgental,Renata Dornelles
Lima,Regina Karla de Pontes
Bonetti-Filho,Idomeo
author_sort Farac,Roberta Vieira
title Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis
title_short Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis
title_full Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis
title_fullStr Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis
title_full_unstemmed Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis
title_sort ex-vivo effect of intracanal medications based on ozone and calcium hydroxide in root canals contaminated with enterococcus faecalis
description This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.
publisher Fundação Odontológica de Ribeirão Preto
publishDate 2013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000200103
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