Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol medium
The aim of this work was to develop a quick direct analytical technique for the determination of oleic acid content in soybean oil by non-aqueous capillary electrophoresis with capacitively coupled contactless conductivity detection. The oil-miscible background electrolyte was a mixture of methanol/1-propanol (1:6 v/v) containing 4 × 10-2 mol L-1 KOH and 10% (v/v) ethylene glycol. Samples of 50 g L-1 soybean oil were prepared directly in the background electrolyte added with 1.33 × 10-3 g L-1 of salicylic acid as internal standard. Quantitative tests were performed by adding to the samples pure oleic acid in the range from 0.53 to 2.13 × 10-3 mol L-1. Under negative polarity anionic solutes moved faster than the electro-osmotic flow so that oleic acid was detected in 16 minutes. The limits of detection and quantification were, respectively, 24 and 81 µmol L-1. Such results demonstrate that, unlike required by other methods, low levels of oleic acid can be quantitatively determined in soybean oil without prior extraction.
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Sociedade Brasileira de Química
2014
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oai:scielo:S0103-505320140009000162014-09-30Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol mediumBöckel,Wolmir JoséSilva,Yara Patrícia daMendonça,Carla R. B.Simó-Alfonso,Ernesto F.Ramis-Ramos,GuillermoPiatnicki,Clarisse M. S. oleic acid non-aqueous capillary electrophoresis (NACE) contactless capacitively coupled contactless conductivity detection (C4D) soybean oil The aim of this work was to develop a quick direct analytical technique for the determination of oleic acid content in soybean oil by non-aqueous capillary electrophoresis with capacitively coupled contactless conductivity detection. The oil-miscible background electrolyte was a mixture of methanol/1-propanol (1:6 v/v) containing 4 × 10-2 mol L-1 KOH and 10% (v/v) ethylene glycol. Samples of 50 g L-1 soybean oil were prepared directly in the background electrolyte added with 1.33 × 10-3 g L-1 of salicylic acid as internal standard. Quantitative tests were performed by adding to the samples pure oleic acid in the range from 0.53 to 2.13 × 10-3 mol L-1. Under negative polarity anionic solutes moved faster than the electro-osmotic flow so that oleic acid was detected in 16 minutes. The limits of detection and quantification were, respectively, 24 and 81 µmol L-1. Such results demonstrate that, unlike required by other methods, low levels of oleic acid can be quantitatively determined in soybean oil without prior extraction.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society v.25 n.9 20142014-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900016en10.5935/0103-5053.20140155 |
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Böckel,Wolmir José Silva,Yara Patrícia da Mendonça,Carla R. B. Simó-Alfonso,Ernesto F. Ramis-Ramos,Guillermo Piatnicki,Clarisse M. S. |
spellingShingle |
Böckel,Wolmir José Silva,Yara Patrícia da Mendonça,Carla R. B. Simó-Alfonso,Ernesto F. Ramis-Ramos,Guillermo Piatnicki,Clarisse M. S. Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol medium |
author_facet |
Böckel,Wolmir José Silva,Yara Patrícia da Mendonça,Carla R. B. Simó-Alfonso,Ernesto F. Ramis-Ramos,Guillermo Piatnicki,Clarisse M. S. |
author_sort |
Böckel,Wolmir José |
title |
Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol medium |
title_short |
Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol medium |
title_full |
Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol medium |
title_fullStr |
Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol medium |
title_full_unstemmed |
Direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible KOH/1-propanol/methanol medium |
title_sort |
direct determination of oleic acid in soybean oil by capacitively coupled contactless conductivity detection capillary electrophoresis in an oil-miscible koh/1-propanol/methanol medium |
description |
The aim of this work was to develop a quick direct analytical technique for the determination of oleic acid content in soybean oil by non-aqueous capillary electrophoresis with capacitively coupled contactless conductivity detection. The oil-miscible background electrolyte was a mixture of methanol/1-propanol (1:6 v/v) containing 4 × 10-2 mol L-1 KOH and 10% (v/v) ethylene glycol. Samples of 50 g L-1 soybean oil were prepared directly in the background electrolyte added with 1.33 × 10-3 g L-1 of salicylic acid as internal standard. Quantitative tests were performed by adding to the samples pure oleic acid in the range from 0.53 to 2.13 × 10-3 mol L-1. Under negative polarity anionic solutes moved faster than the electro-osmotic flow so that oleic acid was detected in 16 minutes. The limits of detection and quantification were, respectively, 24 and 81 µmol L-1. Such results demonstrate that, unlike required by other methods, low levels of oleic acid can be quantitatively determined in soybean oil without prior extraction. |
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Sociedade Brasileira de Química |
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2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900016 |
work_keys_str_mv |
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