Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials
Ergosterol was used as indicator of fungal contamination to assess mould presence on the building materials. Microwave-assisted saponification followed by pentane extraction was used in the sample preparation step. Samples of brick, plaster, concrete and granite were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Ergosterol concentrations in laboratory infected samples ranged from 6 to 16 ng g-1, while in samples of naturally occurring materials ranged from 66 to 3020 ng g-1. Limits of detection and of quantitation were 1.7 and 5.5 ng g-1, respectively. Recovery and matrix effect were tested using samples spiked at 100 ng g-1. Mean recovery and mean matrix effect were 65.9 and 111.6%, respectively. Microscopic observations and bioluminescence measurements were carried out for the infected samples to make additional confirmation of fungi presence. The ergosterol concentration determined by HPLC-MS/MS in building materials proved to be a suitable indicator to estimate fungal contamination. The developed method was considerably faster for this aim than bioluminescence measurements and traditionally used culture methods.
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Sociedade Brasileira de Química
2013
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oai:scielo:S0103-505320130009000122013-09-24Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materialsHorbik,DariaŁowińska-Kluge,AldonaGórski,ZbigniewStanisz,EwaZgoła-Grześkowiak,Agnieszka ergosterol, building material microwave extraction bioluminescence imaging Ergosterol was used as indicator of fungal contamination to assess mould presence on the building materials. Microwave-assisted saponification followed by pentane extraction was used in the sample preparation step. Samples of brick, plaster, concrete and granite were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Ergosterol concentrations in laboratory infected samples ranged from 6 to 16 ng g-1, while in samples of naturally occurring materials ranged from 66 to 3020 ng g-1. Limits of detection and of quantitation were 1.7 and 5.5 ng g-1, respectively. Recovery and matrix effect were tested using samples spiked at 100 ng g-1. Mean recovery and mean matrix effect were 65.9 and 111.6%, respectively. Microscopic observations and bioluminescence measurements were carried out for the infected samples to make additional confirmation of fungi presence. The ergosterol concentration determined by HPLC-MS/MS in building materials proved to be a suitable indicator to estimate fungal contamination. The developed method was considerably faster for this aim than bioluminescence measurements and traditionally used culture methods.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society v.24 n.9 20132013-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000900012en10.5935/0103-5053.20130191 |
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Horbik,Daria Łowińska-Kluge,Aldona Górski,Zbigniew Stanisz,Ewa Zgoła-Grześkowiak,Agnieszka |
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Horbik,Daria Łowińska-Kluge,Aldona Górski,Zbigniew Stanisz,Ewa Zgoła-Grześkowiak,Agnieszka Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
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Horbik,Daria Łowińska-Kluge,Aldona Górski,Zbigniew Stanisz,Ewa Zgoła-Grześkowiak,Agnieszka |
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Horbik,Daria |
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Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
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Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
title_full |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
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Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
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Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
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microwave-assisted extraction combined with hplc-ms/ms for diagnosis of fungal contamination in building materials |
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Ergosterol was used as indicator of fungal contamination to assess mould presence on the building materials. Microwave-assisted saponification followed by pentane extraction was used in the sample preparation step. Samples of brick, plaster, concrete and granite were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Ergosterol concentrations in laboratory infected samples ranged from 6 to 16 ng g-1, while in samples of naturally occurring materials ranged from 66 to 3020 ng g-1. Limits of detection and of quantitation were 1.7 and 5.5 ng g-1, respectively. Recovery and matrix effect were tested using samples spiked at 100 ng g-1. Mean recovery and mean matrix effect were 65.9 and 111.6%, respectively. Microscopic observations and bioluminescence measurements were carried out for the infected samples to make additional confirmation of fungi presence. The ergosterol concentration determined by HPLC-MS/MS in building materials proved to be a suitable indicator to estimate fungal contamination. The developed method was considerably faster for this aim than bioluminescence measurements and traditionally used culture methods. |
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Sociedade Brasileira de Química |
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2013 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000900012 |
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