Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection
This work presents a simple, fast and low-cost method for determining sildenafil citrate (SC) in Viagra® and in similar, generic and manipulated pharmaceutical formulations using flow injection analysis (FIA) with multiple pulse amperometric detection. The method consists on the application of three sequential potential pulses as a function of time. SC is detected at 1.6 and 1.9 V by two different irreversible oxidation processes. The third potential pulse (1.0 V) is applied for the regeneration (cleaning) of the surface of the boron-doped electrode. The limit of detection on an order of 10 nmol L-1 was reached with a relative standard deviation of less than 0.2% (n = 10) for 4.5 X 10-5 mol L-1 CS. The analytical frequency was calculated in 86 injections per hour. The addition recovery studies in all the samples were approximately 100% and the results were validated by chromatographic methods.
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Sociedade Brasileira de Química
2012
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oai:scielo:S0103-505320120010000062012-12-05Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detectionLopes Júnior,Antônio Carlos V.Luz,Rita de Cássia SilvaDamos,Flávio S.Santos,Alexandre S. dosFranco,Diego L.Santos,Wallans T. Pio dos sildenafil citrate multiple pulse amperometry flow injection analysis This work presents a simple, fast and low-cost method for determining sildenafil citrate (SC) in Viagra® and in similar, generic and manipulated pharmaceutical formulations using flow injection analysis (FIA) with multiple pulse amperometric detection. The method consists on the application of three sequential potential pulses as a function of time. SC is detected at 1.6 and 1.9 V by two different irreversible oxidation processes. The third potential pulse (1.0 V) is applied for the regeneration (cleaning) of the surface of the boron-doped electrode. The limit of detection on an order of 10 nmol L-1 was reached with a relative standard deviation of less than 0.2% (n = 10) for 4.5 X 10-5 mol L-1 CS. The analytical frequency was calculated in 86 injections per hour. The addition recovery studies in all the samples were approximately 100% and the results were validated by chromatographic methods.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society v.23 n.10 20122012-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001000006en10.1590/S0103-50532012005000047 |
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Lopes Júnior,Antônio Carlos V. Luz,Rita de Cássia Silva Damos,Flávio S. Santos,Alexandre S. dos Franco,Diego L. Santos,Wallans T. Pio dos |
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Lopes Júnior,Antônio Carlos V. Luz,Rita de Cássia Silva Damos,Flávio S. Santos,Alexandre S. dos Franco,Diego L. Santos,Wallans T. Pio dos Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection |
author_facet |
Lopes Júnior,Antônio Carlos V. Luz,Rita de Cássia Silva Damos,Flávio S. Santos,Alexandre S. dos Franco,Diego L. Santos,Wallans T. Pio dos |
author_sort |
Lopes Júnior,Antônio Carlos V. |
title |
Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection |
title_short |
Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection |
title_full |
Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection |
title_fullStr |
Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection |
title_full_unstemmed |
Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection |
title_sort |
determination of sildenafil citrate (viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection |
description |
This work presents a simple, fast and low-cost method for determining sildenafil citrate (SC) in Viagra® and in similar, generic and manipulated pharmaceutical formulations using flow injection analysis (FIA) with multiple pulse amperometric detection. The method consists on the application of three sequential potential pulses as a function of time. SC is detected at 1.6 and 1.9 V by two different irreversible oxidation processes. The third potential pulse (1.0 V) is applied for the regeneration (cleaning) of the surface of the boron-doped electrode. The limit of detection on an order of 10 nmol L-1 was reached with a relative standard deviation of less than 0.2% (n = 10) for 4.5 X 10-5 mol L-1 CS. The analytical frequency was calculated in 86 injections per hour. The addition recovery studies in all the samples were approximately 100% and the results were validated by chromatographic methods. |
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Sociedade Brasileira de Química |
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2012 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001000006 |
work_keys_str_mv |
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