Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosa
The enzyme UDP-N-acetylglucosamine pyrophosphorylase (PyroMp) from Moniliophthora perniciosa (CCMB 0257), a pathogenic fungal strain and the causative agent of the witches' broom disease in Theobroma cacao, was partially purified by precipitation with ammonium sulfate and gel filtration on Sephacryl S-200. The buffer for enzyme extraction was sodium phosphate, 0.050 mol L-1, pH 7.0, containing 1.0 mol L-1 NaCl. Response surface methodology (RSM) was used to determine the optimum pH and temperature conditions. Four different isoenzymes (PyroMp I, PyroMp II, PyroMp III and PyroMp IV) were obtained with optimal pH ranging from 6.9-8.4 and optimum temperature ranging from 28 to 68 °C. The 3D structure of pyrophosphorylase of M. perniciosa was determined by comparative modeling. The model obtained showed a good quality, possessing 78.6% of amino acids in energetically allowed regions. The model was then submitted for DM simulation and showed a good geometric quality (91.1% Ramachandran plot). The active site of the enzyme was found to be extremely well conserved. This model will be useful for developing new inhibitors against witches' broom disease.
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Sociedade Brasileira de Química
2011
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oai:scielo:S0103-505320110006000032011-06-10Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosaSantos Junior,Manoelito C.Gonçalves,Priscila A.Taranto,Alex G.Koblitz,Maria G. B.Góes-Neto,AristótelesPirovani,Carlos P.Cascardo,Júlio C. M.Cruz,Sandra H. daZingali,Russolina B.Pereira,Gonçalo A. G.Dias,Cristiano V.Assis,Sandra A. de pyrophosphorylase Moniliophthora perniciosa kinetic characterization heat stability 3D structure comparative modeling The enzyme UDP-N-acetylglucosamine pyrophosphorylase (PyroMp) from Moniliophthora perniciosa (CCMB 0257), a pathogenic fungal strain and the causative agent of the witches' broom disease in Theobroma cacao, was partially purified by precipitation with ammonium sulfate and gel filtration on Sephacryl S-200. The buffer for enzyme extraction was sodium phosphate, 0.050 mol L-1, pH 7.0, containing 1.0 mol L-1 NaCl. Response surface methodology (RSM) was used to determine the optimum pH and temperature conditions. Four different isoenzymes (PyroMp I, PyroMp II, PyroMp III and PyroMp IV) were obtained with optimal pH ranging from 6.9-8.4 and optimum temperature ranging from 28 to 68 °C. The 3D structure of pyrophosphorylase of M. perniciosa was determined by comparative modeling. The model obtained showed a good quality, possessing 78.6% of amino acids in energetically allowed regions. The model was then submitted for DM simulation and showed a good geometric quality (91.1% Ramachandran plot). The active site of the enzyme was found to be extremely well conserved. This model will be useful for developing new inhibitors against witches' broom disease.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society v.22 n.6 20112011-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000600003en10.1590/S0103-50532011000600003 |
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Santos Junior,Manoelito C. Gonçalves,Priscila A. Taranto,Alex G. Koblitz,Maria G. B. Góes-Neto,Aristóteles Pirovani,Carlos P. Cascardo,Júlio C. M. Cruz,Sandra H. da Zingali,Russolina B. Pereira,Gonçalo A. G. Dias,Cristiano V. Assis,Sandra A. de |
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Santos Junior,Manoelito C. Gonçalves,Priscila A. Taranto,Alex G. Koblitz,Maria G. B. Góes-Neto,Aristóteles Pirovani,Carlos P. Cascardo,Júlio C. M. Cruz,Sandra H. da Zingali,Russolina B. Pereira,Gonçalo A. G. Dias,Cristiano V. Assis,Sandra A. de Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosa |
author_facet |
Santos Junior,Manoelito C. Gonçalves,Priscila A. Taranto,Alex G. Koblitz,Maria G. B. Góes-Neto,Aristóteles Pirovani,Carlos P. Cascardo,Júlio C. M. Cruz,Sandra H. da Zingali,Russolina B. Pereira,Gonçalo A. G. Dias,Cristiano V. Assis,Sandra A. de |
author_sort |
Santos Junior,Manoelito C. |
title |
Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosa |
title_short |
Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosa |
title_full |
Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosa |
title_fullStr |
Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosa |
title_full_unstemmed |
Purification, characterization and structural determination of UDP-N-acetylglucosamine pyrophosphorylase produced by Moniliophthora perniciosa |
title_sort |
purification, characterization and structural determination of udp-n-acetylglucosamine pyrophosphorylase produced by moniliophthora perniciosa |
description |
The enzyme UDP-N-acetylglucosamine pyrophosphorylase (PyroMp) from Moniliophthora perniciosa (CCMB 0257), a pathogenic fungal strain and the causative agent of the witches' broom disease in Theobroma cacao, was partially purified by precipitation with ammonium sulfate and gel filtration on Sephacryl S-200. The buffer for enzyme extraction was sodium phosphate, 0.050 mol L-1, pH 7.0, containing 1.0 mol L-1 NaCl. Response surface methodology (RSM) was used to determine the optimum pH and temperature conditions. Four different isoenzymes (PyroMp I, PyroMp II, PyroMp III and PyroMp IV) were obtained with optimal pH ranging from 6.9-8.4 and optimum temperature ranging from 28 to 68 °C. The 3D structure of pyrophosphorylase of M. perniciosa was determined by comparative modeling. The model obtained showed a good quality, possessing 78.6% of amino acids in energetically allowed regions. The model was then submitted for DM simulation and showed a good geometric quality (91.1% Ramachandran plot). The active site of the enzyme was found to be extremely well conserved. This model will be useful for developing new inhibitors against witches' broom disease. |
publisher |
Sociedade Brasileira de Química |
publishDate |
2011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000600003 |
work_keys_str_mv |
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