Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications
An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the determination of gemifloxacin in bulk, dosage formulations and human serum at 270 nm. Chromatographic separation was achieved on Purospher STAR C18 (250 × 4.6 mm, 5 µm) column using mobile phase methanol:water (90:10, v/v) adjusted pH 2.8 via phosphoric acid 85% having flow rate of 1.5 mL min-1 at ambient temperature. Calibration curves were linear over range of 5-100 µg mL-1 with a correlation coefficient 0.9998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.015 and 0.045 µg mL-1, respectively. Intra and inter-run precision and accuracy results were 98.73-100.12% and then correlated through student'st-test. This method was further applied for in vitro interactions of gemifloxacin with essential and trace elements.
Main Authors: | , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Química
2011
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000500024 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0103-50532011000500024 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0103-505320110005000242011-05-16Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applicationsSultana,NajmaArayne,M. SaeedShamim,SanaAkhtar,MahwishGul,Somia gemifloxacin HPLC human serum student's t-test An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the determination of gemifloxacin in bulk, dosage formulations and human serum at 270 nm. Chromatographic separation was achieved on Purospher STAR C18 (250 × 4.6 mm, 5 µm) column using mobile phase methanol:water (90:10, v/v) adjusted pH 2.8 via phosphoric acid 85% having flow rate of 1.5 mL min-1 at ambient temperature. Calibration curves were linear over range of 5-100 µg mL-1 with a correlation coefficient 0.9998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.015 and 0.045 µg mL-1, respectively. Intra and inter-run precision and accuracy results were 98.73-100.12% and then correlated through student'st-test. This method was further applied for in vitro interactions of gemifloxacin with essential and trace elements.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society v.22 n.5 20112011-05-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000500024en10.1590/S0103-50532011000500024 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Sultana,Najma Arayne,M. Saeed Shamim,Sana Akhtar,Mahwish Gul,Somia |
spellingShingle |
Sultana,Najma Arayne,M. Saeed Shamim,Sana Akhtar,Mahwish Gul,Somia Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications |
author_facet |
Sultana,Najma Arayne,M. Saeed Shamim,Sana Akhtar,Mahwish Gul,Somia |
author_sort |
Sultana,Najma |
title |
Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications |
title_short |
Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications |
title_full |
Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications |
title_fullStr |
Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications |
title_full_unstemmed |
Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications |
title_sort |
validated method for the determination of gemifloxacin in bulk, pharmaceutical formulations and human serum by rp-hplc: in vitro applications |
description |
An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the determination of gemifloxacin in bulk, dosage formulations and human serum at 270 nm. Chromatographic separation was achieved on Purospher STAR C18 (250 × 4.6 mm, 5 µm) column using mobile phase methanol:water (90:10, v/v) adjusted pH 2.8 via phosphoric acid 85% having flow rate of 1.5 mL min-1 at ambient temperature. Calibration curves were linear over range of 5-100 µg mL-1 with a correlation coefficient 0.9998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.015 and 0.045 µg mL-1, respectively. Intra and inter-run precision and accuracy results were 98.73-100.12% and then correlated through student'st-test. This method was further applied for in vitro interactions of gemifloxacin with essential and trace elements. |
publisher |
Sociedade Brasileira de Química |
publishDate |
2011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000500024 |
work_keys_str_mv |
AT sultananajma validatedmethodforthedeterminationofgemifloxacininbulkpharmaceuticalformulationsandhumanserumbyrphplcinvitroapplications AT araynemsaeed validatedmethodforthedeterminationofgemifloxacininbulkpharmaceuticalformulationsandhumanserumbyrphplcinvitroapplications AT shamimsana validatedmethodforthedeterminationofgemifloxacininbulkpharmaceuticalformulationsandhumanserumbyrphplcinvitroapplications AT akhtarmahwish validatedmethodforthedeterminationofgemifloxacininbulkpharmaceuticalformulationsandhumanserumbyrphplcinvitroapplications AT gulsomia validatedmethodforthedeterminationofgemifloxacininbulkpharmaceuticalformulationsandhumanserumbyrphplcinvitroapplications |
_version_ |
1756403436053594112 |