HPLC analysis of glycosylated amino acids
This work describes the standardization of HPLC analysis methodology for the identification and separation of the glycosylated amino acids (building blocks) 3, 4, 7 and 8 and their respective precursors. The glycosylated amino acids containing the sugar units beta-D-GlcNAc (3 and 7) O-linked to threonine amino acids are involved in cellular signalling events, and the correspondent isomers 4 and 8, possessing alpha-D-GlcNAc units are related to O-glycans found in mucins of the parasite Trypanosoma cruzi. The developed methodology is advantageous and efficient as it permits the analysis of crude reaction mixtures, avoiding exhaustive purification processes.
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Main Authors: | Campo,Vanessa L., Borges,Áurea D. L., Carvalho,Ivone |
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Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Química
2006
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000400004 |
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