Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients
ABSTRACT Purpose As a classical xenotransplantation model, porcine kidneys have been transplanted into the lower abdomen of non-human primates. However, we have improved upon this model by using size-matched grafting in the orthotopic position. The beneficial aspects and surgical details of our method are reported herein. Methods Donors were two newborn pigs (weighting 5 to 6 kg) and recipients were two cynomolgus monkeys (weighting, approximately, 7 kg). After bilateral nephrectomy, kidneys were cold-transported in Euro-Collins solution. The porcine kidney was transplanted to the site of a left nephrectomy and fixed to the peritoneum. Results Kidneys transplanted to the lower abdomen by the conventional method were more susceptible to torsion of the renal vein (two cases). In contrast, early-stage blood flow insufficiency did not occur in orthotopic transplants of theleft kidney. Conclusions Size-matched porcine-primate renal grafting using our method of transplanting tothe natural position of the kidneys contributes to stable post-transplant blood flow to the kidney.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2021
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oai:scielo:S0102-865020210005002032021-06-16Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipientsTakamura,TsuyoshiSasaki,HiroshiHirayama,HaruyukiKiyoshi,AkihikoInoue,MakotoMatsui,KenjiMatsumoto,NaotoSaito,YatsumuFujimoto,ToshinariTajiri,SusumuYamanaka,ShuichiroMatsumoto,KeiMiyawaki,TakeshiYokoo,TakashiKobayashi,Eiji Kidney Transplantation Surgery Swine Primates ABSTRACT Purpose As a classical xenotransplantation model, porcine kidneys have been transplanted into the lower abdomen of non-human primates. However, we have improved upon this model by using size-matched grafting in the orthotopic position. The beneficial aspects and surgical details of our method are reported herein. Methods Donors were two newborn pigs (weighting 5 to 6 kg) and recipients were two cynomolgus monkeys (weighting, approximately, 7 kg). After bilateral nephrectomy, kidneys were cold-transported in Euro-Collins solution. The porcine kidney was transplanted to the site of a left nephrectomy and fixed to the peritoneum. Results Kidneys transplanted to the lower abdomen by the conventional method were more susceptible to torsion of the renal vein (two cases). In contrast, early-stage blood flow insufficiency did not occur in orthotopic transplants of theleft kidney. Conclusions Size-matched porcine-primate renal grafting using our method of transplanting tothe natural position of the kidneys contributes to stable post-transplant blood flow to the kidney.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.36 n.5 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000500203en10.1590/acb360503 |
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Takamura,Tsuyoshi Sasaki,Hiroshi Hirayama,Haruyuki Kiyoshi,Akihiko Inoue,Makoto Matsui,Kenji Matsumoto,Naoto Saito,Yatsumu Fujimoto,Toshinari Tajiri,Susumu Yamanaka,Shuichiro Matsumoto,Kei Miyawaki,Takeshi Yokoo,Takashi Kobayashi,Eiji |
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Takamura,Tsuyoshi Sasaki,Hiroshi Hirayama,Haruyuki Kiyoshi,Akihiko Inoue,Makoto Matsui,Kenji Matsumoto,Naoto Saito,Yatsumu Fujimoto,Toshinari Tajiri,Susumu Yamanaka,Shuichiro Matsumoto,Kei Miyawaki,Takeshi Yokoo,Takashi Kobayashi,Eiji Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients |
author_facet |
Takamura,Tsuyoshi Sasaki,Hiroshi Hirayama,Haruyuki Kiyoshi,Akihiko Inoue,Makoto Matsui,Kenji Matsumoto,Naoto Saito,Yatsumu Fujimoto,Toshinari Tajiri,Susumu Yamanaka,Shuichiro Matsumoto,Kei Miyawaki,Takeshi Yokoo,Takashi Kobayashi,Eiji |
author_sort |
Takamura,Tsuyoshi |
title |
Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients |
title_short |
Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients |
title_full |
Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients |
title_fullStr |
Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients |
title_full_unstemmed |
Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients |
title_sort |
techniques of orthotopic renal transplantation. ii. size-matched porcine grafts in monkey recipients |
description |
ABSTRACT Purpose As a classical xenotransplantation model, porcine kidneys have been transplanted into the lower abdomen of non-human primates. However, we have improved upon this model by using size-matched grafting in the orthotopic position. The beneficial aspects and surgical details of our method are reported herein. Methods Donors were two newborn pigs (weighting 5 to 6 kg) and recipients were two cynomolgus monkeys (weighting, approximately, 7 kg). After bilateral nephrectomy, kidneys were cold-transported in Euro-Collins solution. The porcine kidney was transplanted to the site of a left nephrectomy and fixed to the peritoneum. Results Kidneys transplanted to the lower abdomen by the conventional method were more susceptible to torsion of the renal vein (two cases). In contrast, early-stage blood flow insufficiency did not occur in orthotopic transplants of theleft kidney. Conclusions Size-matched porcine-primate renal grafting using our method of transplanting tothe natural position of the kidneys contributes to stable post-transplant blood flow to the kidney. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publishDate |
2021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000500203 |
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