Tissue content of metalloproteinase-9 and collagen in the colon with and without fecal stream after intervention with infliximab in rats subjected to Hartmann’s surgery
ABSTRACT Purpose Quantify the tissue content of metalloproteinase-9 (MMP-9) and collagen in colic mucosa with and without intestinal transit after infliximab administration in rats subjected to Hartmann’s surgery. Methods Twenty-two rats underwent colon diversion by Hartmann’s surgery. Animals were maintained with intestinal bypass for 12 weeks to induce development of diversion colitis (DC). Afterwards, animals were divided into three groups: first group received subcutaneous application of saline solution (SS) 0.9%, while the remaining two groups received infliximab subcutaneously at doses of 5 or 10 mg·kg–1·week–1 for five consecutive weeks. After the intervention, animals were sacrificed, removing the segments with and without intestinal transit. Diversion colitis was diagnosed by histological study, and its intensity was determined by a validated inflammatory scale. Tissue expression of MMP-9 was assessed byimmunohistochemistry, while total collagen was assessed by histochemistry. Tissue content of both was measuredby computerized morphometry. Results Colon segments without intestinal transit had a higher degree of inflammation, which improved in animals treated with infliximab. Collagen content was always lower in those without intestinal transit. There was an increase in the collagen content in the colon without transit in animals treated with infliximab, primarily at a dose of 10 mg·kg–1·week–1. There was an increase in the content of MMP-9 in the colon without fecal transit, and a reduction was observed in animals treated with infliximab, regardless of the dose used. Conclusions Application of infliximab reduces inflammation, increases the total collagen content and decreases the content of MMP-9 in the colon without intestinal transit.
Main Authors: | Alves Junior,Antonio José Tiburcio, Pereira,José Aires, Ávila,Mariane Grandi de, Domingues,Fernanda Aparecida, Sato,Daniela Tiemi, Martinez,Carlos Augusto Real |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2021
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000400201 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate
by: Bonassa,Christiny Emmanuelle Gabriel, et al.
Published: (2015) -
Tissue quantification of neutral and acid mucins in the mucosa of the colon with and without fecal stream in rats
by: Nonose,Ronaldo, et al.
Published: (2009) -
Sucralfate enemas reduce the oxidative tissue damage and preserves the contents of E-cadherin and Β-catenin in colonic mucosa without fecal stream
by: Sato,Daniela Tiemi, et al.
Published: (2021) -
Reduction in oxidative stress levels in the colonic mucosa without fecal stream after the application of enemas containing aqueous Ilex paraguariensis extract
by: Cunha,Fernando Lorenzetti da, et al.
Published: (2011) -
Influence of the application of enemas with oily extract of curcumin (Curcuma longa) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream
by: Martinez,Carlos Augusto Real, et al.
Published: (2017)