Characterization of the intestinal graft in a swine hypotensive after brain death model
Abstract Purpose: To establish a hypotensive brain death pig model and observe the effects of hypotension on small bowel donors. Methods: The hypotensive brain death model was produced using the modified intracranial water sac inflation method in ten domestic crossbred pigs. Effects of hypotensive brain death on small bowel tissue morphology were evaluated through changes in intestinal tissue pathology, tight junction protein of the intestinal mucosa and plasma intestinal fatty acid-binding protein (i-FABP) levels. The pathophysiological mechanism was examined based on changes in superior mesenteric artery (SMA) blood flow and systemic hemodynamics. Results: After model establishment, SMA blood flow, and the mean arterial pressure (MAP) significantly decreased, while heart rate increased rapidly and fluctuated significantly. Small bowel tissue morphology and levels of tight junction protein of the intestinal mucosa showed that after model establishment, small bowel tissue injury was gradually aggravated over time (P<0.05). Plasma i-FABP levels significantly increased after brain death (P<0.05). Conclusions: A hypotensive brain death pig model was successfully established using an improved intracranial water sac inflation method. This method offers a possibility of describing the injury mechanisms more clearly during and after brain death.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2019
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oai:scielo:S0102-865020190011002142019-12-19Characterization of the intestinal graft in a swine hypotensive after brain death modelLi,LinlinGao,YingLu,ChunleiGuo,Mingxiao Brain Death Hypotensive Swine Abstract Purpose: To establish a hypotensive brain death pig model and observe the effects of hypotension on small bowel donors. Methods: The hypotensive brain death model was produced using the modified intracranial water sac inflation method in ten domestic crossbred pigs. Effects of hypotensive brain death on small bowel tissue morphology were evaluated through changes in intestinal tissue pathology, tight junction protein of the intestinal mucosa and plasma intestinal fatty acid-binding protein (i-FABP) levels. The pathophysiological mechanism was examined based on changes in superior mesenteric artery (SMA) blood flow and systemic hemodynamics. Results: After model establishment, SMA blood flow, and the mean arterial pressure (MAP) significantly decreased, while heart rate increased rapidly and fluctuated significantly. Small bowel tissue morphology and levels of tight junction protein of the intestinal mucosa showed that after model establishment, small bowel tissue injury was gradually aggravated over time (P<0.05). Plasma i-FABP levels significantly increased after brain death (P<0.05). Conclusions: A hypotensive brain death pig model was successfully established using an improved intracranial water sac inflation method. This method offers a possibility of describing the injury mechanisms more clearly during and after brain death.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.34 n.11 20192019-11-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019001100214en10.1590/s0102-865020190110000007 |
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Li,Linlin Gao,Ying Lu,Chunlei Guo,Mingxiao |
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Li,Linlin Gao,Ying Lu,Chunlei Guo,Mingxiao Characterization of the intestinal graft in a swine hypotensive after brain death model |
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Li,Linlin Gao,Ying Lu,Chunlei Guo,Mingxiao |
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Li,Linlin |
title |
Characterization of the intestinal graft in a swine hypotensive after brain death model |
title_short |
Characterization of the intestinal graft in a swine hypotensive after brain death model |
title_full |
Characterization of the intestinal graft in a swine hypotensive after brain death model |
title_fullStr |
Characterization of the intestinal graft in a swine hypotensive after brain death model |
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Characterization of the intestinal graft in a swine hypotensive after brain death model |
title_sort |
characterization of the intestinal graft in a swine hypotensive after brain death model |
description |
Abstract Purpose: To establish a hypotensive brain death pig model and observe the effects of hypotension on small bowel donors. Methods: The hypotensive brain death model was produced using the modified intracranial water sac inflation method in ten domestic crossbred pigs. Effects of hypotensive brain death on small bowel tissue morphology were evaluated through changes in intestinal tissue pathology, tight junction protein of the intestinal mucosa and plasma intestinal fatty acid-binding protein (i-FABP) levels. The pathophysiological mechanism was examined based on changes in superior mesenteric artery (SMA) blood flow and systemic hemodynamics. Results: After model establishment, SMA blood flow, and the mean arterial pressure (MAP) significantly decreased, while heart rate increased rapidly and fluctuated significantly. Small bowel tissue morphology and levels of tight junction protein of the intestinal mucosa showed that after model establishment, small bowel tissue injury was gradually aggravated over time (P<0.05). Plasma i-FABP levels significantly increased after brain death (P<0.05). Conclusions: A hypotensive brain death pig model was successfully established using an improved intracranial water sac inflation method. This method offers a possibility of describing the injury mechanisms more clearly during and after brain death. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2019 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019001100214 |
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AT lilinlin characterizationoftheintestinalgraftinaswinehypotensiveafterbraindeathmodel AT gaoying characterizationoftheintestinalgraftinaswinehypotensiveafterbraindeathmodel AT luchunlei characterizationoftheintestinalgraftinaswinehypotensiveafterbraindeathmodel AT guomingxiao characterizationoftheintestinalgraftinaswinehypotensiveafterbraindeathmodel |
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