Perconditioning combined with postconditioning on kidney ischemia and reperfusion

Abstract Purpose: To evaluate if combination of perconditioning and postconditioning provides improved renal protection compared to perconditioning alone in a model of renal reperfusion injury. Methods: Thirty rats were assigned into 6 groups: normality; sham; ischemia and reperfusion; postconditioning; perconditioning; perconditioning + postconditioning. Animals were subjected to right nephrectomy and left renal ischemia for 30 minutes. Postconditioning consisted of 3 cycles of 5 min renal perfusion followed by 5 min of renal ischemia after major ischemic period. Perconditioning consisted of 3 cycles of 5 min hindlimb ischemia followed by 5 min of hindlimb perfusion contemporaneously to renal major ischemic period. After 24 hours, kidney was harvested and blood collected to measure urea and creatinine. Results: Perconditioning obtained better values for creatinine and urea level than only postconditioning (p<0.01); performing both techniques contemporaneously had no increased results (p>0.05). Regarding tissue structure, perconditioning was the only technique to protect the glomerulus and tubules (p<0.05), while postconditioning protected only the glomerulus (p<0.05). Combination of both techniques shows no effect on glomerulus or tubules (p>0.05). Conclusions: Perconditioning had promising results on ischemia and reperfusion induced kidney injury, enhanced kidney function and protected glomerulus and tubules. There was no additive protection when postconditioning and perconditioning were combined.

Bibliographic Details

Main Authors:	Costa,Felipe Lobato da Silva, Yamaki,Vitor Nagai, Teixeira,Renan Kleber Costa, Feijó,Daniel Haber, Valente,André Lopes, Carvalho,Luan Teles Ferreira de, Yasojima,Edson Yuzur, Brito,Marcus Vinicius Henriques
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2017
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000800599
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