Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing
ABSTRACT PURPOSE: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process. METHODS: L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration. RESULTS: Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-β and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-β expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice. CONCLUSION: L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2016
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oai:scielo:S0102-865020160009005862016-10-05Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healingJerônimo,Márcio SousaBarros,Adria do PradoMoritaI,Vinícius Elord ZenAlves,Erika OliveiraSouza,Nathalia Lobão Barroso deAlmeida,Rosane Mansan deNóbrega,Yanna Karla MedeirosCavalcanti Neto,Florêncio FigueiredoAmorin,RivadávioBorin,Maria de FátimaBocca,Anamélia Lorenzetti Arginine Wound Healing Transforming Growth Factor beta Nitric Oxide Cytokines Mice ABSTRACT PURPOSE: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process. METHODS: L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration. RESULTS: Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-β and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-β expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice. CONCLUSION: L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.31 n.9 20162016-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000900586en10.1590/S0102-865020160090000003 |
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Jerônimo,Márcio Sousa Barros,Adria do Prado MoritaI,Vinícius Elord Zen Alves,Erika Oliveira Souza,Nathalia Lobão Barroso de Almeida,Rosane Mansan de Nóbrega,Yanna Karla Medeiros Cavalcanti Neto,Florêncio Figueiredo Amorin,Rivadávio Borin,Maria de Fátima Bocca,Anamélia Lorenzetti |
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Jerônimo,Márcio Sousa Barros,Adria do Prado MoritaI,Vinícius Elord Zen Alves,Erika Oliveira Souza,Nathalia Lobão Barroso de Almeida,Rosane Mansan de Nóbrega,Yanna Karla Medeiros Cavalcanti Neto,Florêncio Figueiredo Amorin,Rivadávio Borin,Maria de Fátima Bocca,Anamélia Lorenzetti Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing |
author_facet |
Jerônimo,Márcio Sousa Barros,Adria do Prado MoritaI,Vinícius Elord Zen Alves,Erika Oliveira Souza,Nathalia Lobão Barroso de Almeida,Rosane Mansan de Nóbrega,Yanna Karla Medeiros Cavalcanti Neto,Florêncio Figueiredo Amorin,Rivadávio Borin,Maria de Fátima Bocca,Anamélia Lorenzetti |
author_sort |
Jerônimo,Márcio Sousa |
title |
Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing |
title_short |
Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing |
title_full |
Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing |
title_fullStr |
Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing |
title_full_unstemmed |
Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing |
title_sort |
oral or topical administration of l-arginine changes the expression of tgf and inos and results in early wounds healing |
description |
ABSTRACT PURPOSE: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process. METHODS: L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration. RESULTS: Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-β and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-β expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice. CONCLUSION: L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues. |
publisher |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publishDate |
2016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000900586 |
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