Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro
PURPOSE: To detect whether chitin and sepia ink sponge (CS) can promote wound healing and elevate impact of CS on phagocytosis ability of macrophages. METHODS: Forty-eight rats were assigned to four groups: Normal group (Normal), negative control group (Con), chitin and sepia ink sponge group (CS) and positive control Surgicel Gauze(r) group (SG). Deep second-degree burn model was created in rats. Wound area was recorded by digital imaging and determined using Image J software. Samples were collected and kept at -80oC on 3d, 7d, 14d and 21d for cytokines detecting. Transforming growth factor (TGF)-β1, interleukin (IL)-6, matrix metalloproteinase (MMP)-1, hydroxyproline (Hyp) and macrophage activity reflected by tumor necrosis factor (TNF)-α were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Comparing to Con and SG, scabs in CS group fell off and basically healed on 21 day. TGF-β1, IL-6, MMP-1 and Hyp were significantly increased by CS and SG comparing to Con (p < 0.05), CS had more apparently adjustment on TGF-β1 and MMP-1 compared to SG; results in vitro indicated CS significantly promoted phagocytosis ability of macrophages reflected in TNF-α (p < 0.05). CONCLUSION: CS improved wound healing through exerting significant influences on secretion of kinds of cytokines and activating macrophages.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2016
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oai:scielo:S0102-865020160002001192016-03-03Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitroZhang,WeiWang,YanSui,XianxianSun,YulinChen,Daohai Chitin Sepia Cytokines Macrophages Rats PURPOSE: To detect whether chitin and sepia ink sponge (CS) can promote wound healing and elevate impact of CS on phagocytosis ability of macrophages. METHODS: Forty-eight rats were assigned to four groups: Normal group (Normal), negative control group (Con), chitin and sepia ink sponge group (CS) and positive control Surgicel Gauze(r) group (SG). Deep second-degree burn model was created in rats. Wound area was recorded by digital imaging and determined using Image J software. Samples were collected and kept at -80oC on 3d, 7d, 14d and 21d for cytokines detecting. Transforming growth factor (TGF)-β1, interleukin (IL)-6, matrix metalloproteinase (MMP)-1, hydroxyproline (Hyp) and macrophage activity reflected by tumor necrosis factor (TNF)-α were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Comparing to Con and SG, scabs in CS group fell off and basically healed on 21 day. TGF-β1, IL-6, MMP-1 and Hyp were significantly increased by CS and SG comparing to Con (p < 0.05), CS had more apparently adjustment on TGF-β1 and MMP-1 compared to SG; results in vitro indicated CS significantly promoted phagocytosis ability of macrophages reflected in TNF-α (p < 0.05). CONCLUSION: CS improved wound healing through exerting significant influences on secretion of kinds of cytokines and activating macrophages.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.31 n.2 20162016-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000200119en10.1590/S0102-865020160020000006 |
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Zhang,Wei Wang,Yan Sui,Xianxian Sun,Yulin Chen,Daohai Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro |
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Zhang,Wei Wang,Yan Sui,Xianxian Sun,Yulin Chen,Daohai |
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Zhang,Wei |
title |
Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro |
title_short |
Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro |
title_full |
Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro |
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Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro |
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Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro |
title_sort |
effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro |
description |
PURPOSE: To detect whether chitin and sepia ink sponge (CS) can promote wound healing and elevate impact of CS on phagocytosis ability of macrophages. METHODS: Forty-eight rats were assigned to four groups: Normal group (Normal), negative control group (Con), chitin and sepia ink sponge group (CS) and positive control Surgicel Gauze(r) group (SG). Deep second-degree burn model was created in rats. Wound area was recorded by digital imaging and determined using Image J software. Samples were collected and kept at -80oC on 3d, 7d, 14d and 21d for cytokines detecting. Transforming growth factor (TGF)-β1, interleukin (IL)-6, matrix metalloproteinase (MMP)-1, hydroxyproline (Hyp) and macrophage activity reflected by tumor necrosis factor (TNF)-α were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Comparing to Con and SG, scabs in CS group fell off and basically healed on 21 day. TGF-β1, IL-6, MMP-1 and Hyp were significantly increased by CS and SG comparing to Con (p < 0.05), CS had more apparently adjustment on TGF-β1 and MMP-1 compared to SG; results in vitro indicated CS significantly promoted phagocytosis ability of macrophages reflected in TNF-α (p < 0.05). CONCLUSION: CS improved wound healing through exerting significant influences on secretion of kinds of cytokines and activating macrophages. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2016 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000200119 |
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