Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine
PURPOSE:To investigate the effect of ischemic preconditioning (IPC) and adenosine as strategies to protect cardiac injury caused by intestinal IR in rats, based on increasing in adenosine bioavailability and improvement of cell energy state by IPC.METHODS: Male Wistar rats were submitted to 60 minutes of intestinal ischemia and 120 minutes of reperfusion. Intravenous injections of saline or Adenosine (AD) was administered five minutes before ischemia, five minutes before reperfusion and after 55 minutes reperfusion. Cardiac samples were obtained, fixed in formalin solution, embedded in paraffin, and sections of 5 μm were stained by hematoxylin-eosin. Histological analysis of myocardium was performed according occurrence of necrosis signs: piknosis, band contraction, eosinophilic cytoplasm, karyorrhexis and vacuolization (score - zero to 5).RESULTS: The groups submitted to ischemia alone (I=4.0), and reperfusion (IR=4.5) showed highest level of lesion compared to the others (I+IPC=3.3, IR+IPC=3.6, I+AD=3.0, IR+AD=3.8). The most interesting result was association of IPC and AD in IR model (IR+IPC+AD=1.2, p=0.002), showing preservation of the heart tissue, with fibers showing typical cross-striations and nuclei characteristics. Rare and small areas of tissue necrosis was observed and suggestion of capillaries congestion.CONCLUSION:Intestinal ischemia reperfusion promotes cardiac tissue injury. Ischemic preconditioning in association with adenosine is an efficient strategy to protect the heart against ischemia and reperfusion injury.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2014
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oai:scielo:S0102-865020140014000142015-10-08Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosineMontero,Micaela FrassonSaurim,RafaelBonservizi,Wesley Guedes SavaKoike,Marcia KiyomiTaha,Murched Omar Adenosine Ischemic preconditioning Intestinal ischemia/reperfusion Heart Rats PURPOSE:To investigate the effect of ischemic preconditioning (IPC) and adenosine as strategies to protect cardiac injury caused by intestinal IR in rats, based on increasing in adenosine bioavailability and improvement of cell energy state by IPC.METHODS: Male Wistar rats were submitted to 60 minutes of intestinal ischemia and 120 minutes of reperfusion. Intravenous injections of saline or Adenosine (AD) was administered five minutes before ischemia, five minutes before reperfusion and after 55 minutes reperfusion. Cardiac samples were obtained, fixed in formalin solution, embedded in paraffin, and sections of 5 μm were stained by hematoxylin-eosin. Histological analysis of myocardium was performed according occurrence of necrosis signs: piknosis, band contraction, eosinophilic cytoplasm, karyorrhexis and vacuolization (score - zero to 5).RESULTS: The groups submitted to ischemia alone (I=4.0), and reperfusion (IR=4.5) showed highest level of lesion compared to the others (I+IPC=3.3, IR+IPC=3.6, I+AD=3.0, IR+AD=3.8). The most interesting result was association of IPC and AD in IR model (IR+IPC+AD=1.2, p=0.002), showing preservation of the heart tissue, with fibers showing typical cross-striations and nuclei characteristics. Rare and small areas of tissue necrosis was observed and suggestion of capillaries congestion.CONCLUSION:Intestinal ischemia reperfusion promotes cardiac tissue injury. Ischemic preconditioning in association with adenosine is an efficient strategy to protect the heart against ischemia and reperfusion injury.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.29 suppl.2 20142014-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400014en10.1590/S0102-8650201400140013 |
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Montero,Micaela Frasson Saurim,Rafael Bonservizi,Wesley Guedes Sava Koike,Marcia Kiyomi Taha,Murched Omar |
spellingShingle |
Montero,Micaela Frasson Saurim,Rafael Bonservizi,Wesley Guedes Sava Koike,Marcia Kiyomi Taha,Murched Omar Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine |
author_facet |
Montero,Micaela Frasson Saurim,Rafael Bonservizi,Wesley Guedes Sava Koike,Marcia Kiyomi Taha,Murched Omar |
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Montero,Micaela Frasson |
title |
Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine |
title_short |
Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine |
title_full |
Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine |
title_fullStr |
Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine |
title_full_unstemmed |
Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine |
title_sort |
heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine |
description |
PURPOSE:To investigate the effect of ischemic preconditioning (IPC) and adenosine as strategies to protect cardiac injury caused by intestinal IR in rats, based on increasing in adenosine bioavailability and improvement of cell energy state by IPC.METHODS: Male Wistar rats were submitted to 60 minutes of intestinal ischemia and 120 minutes of reperfusion. Intravenous injections of saline or Adenosine (AD) was administered five minutes before ischemia, five minutes before reperfusion and after 55 minutes reperfusion. Cardiac samples were obtained, fixed in formalin solution, embedded in paraffin, and sections of 5 μm were stained by hematoxylin-eosin. Histological analysis of myocardium was performed according occurrence of necrosis signs: piknosis, band contraction, eosinophilic cytoplasm, karyorrhexis and vacuolization (score - zero to 5).RESULTS: The groups submitted to ischemia alone (I=4.0), and reperfusion (IR=4.5) showed highest level of lesion compared to the others (I+IPC=3.3, IR+IPC=3.6, I+AD=3.0, IR+AD=3.8). The most interesting result was association of IPC and AD in IR model (IR+IPC+AD=1.2, p=0.002), showing preservation of the heart tissue, with fibers showing typical cross-striations and nuclei characteristics. Rare and small areas of tissue necrosis was observed and suggestion of capillaries congestion.CONCLUSION:Intestinal ischemia reperfusion promotes cardiac tissue injury. Ischemic preconditioning in association with adenosine is an efficient strategy to protect the heart against ischemia and reperfusion injury. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400014 |
work_keys_str_mv |
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