Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs
PURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p < 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2011
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oai:scielo:S0102-865020110008000112011-10-24Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogsWaisberg,JaquesNeff,Charles BenjaminWaisberg,Daniel ReisGermini,DemetriusGonçalves,José EduardoZanotto,ArnaldoSperanzini,Manlio Basilio Islets of Langerhans Transplantation Diabetes Mellitus, Experimental Animal Experimentation Dogs PURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p < 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.26 suppl.2 20112011-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000800011en10.1590/S0102-86502011000800011 |
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Waisberg,Jaques Neff,Charles Benjamin Waisberg,Daniel Reis Germini,Demetrius Gonçalves,José Eduardo Zanotto,Arnaldo Speranzini,Manlio Basilio |
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Waisberg,Jaques Neff,Charles Benjamin Waisberg,Daniel Reis Germini,Demetrius Gonçalves,José Eduardo Zanotto,Arnaldo Speranzini,Manlio Basilio Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
author_facet |
Waisberg,Jaques Neff,Charles Benjamin Waisberg,Daniel Reis Germini,Demetrius Gonçalves,José Eduardo Zanotto,Arnaldo Speranzini,Manlio Basilio |
author_sort |
Waisberg,Jaques |
title |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_short |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_full |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_fullStr |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_full_unstemmed |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_sort |
pancreatic islet allograft in spleen with immunossuppression with cyclosporine. experimental model in dogs |
description |
PURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p < 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000800011 |
work_keys_str_mv |
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1756400896176029696 |