Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry
PURPOSE: To evaluate the effects of biliary stenting in rats through analysis of collagen and elastin deposition in the bile ducts. METHODS: Twenty male rats underwent midline laparotomy, duodenotomy and transampullary stenting of the common bile duct with a 22Fr plastic stent. Animals were randomized in 4 groups, with 5 components in each: (I) control, (II) biliary stenting for 7 days, (III) biliary stenting for 14 days, and (IV) biliary stenting for 30 days. Sections of the common bile duct were stained using Hematoxylin Eosin; Sirius Red to quantitate the amount of collagen present; and Weigert's Resorcin-fuchsin to quantitate the amount of elastin present. RESULTS: The percentage of area stained for collagen was 13.4; 21.5; 29.5 and 32.8, for groups I to IV, respectively. The percentage of area stained for elastin fibers was 7.0; 5.2; 4.0 and 2.9, for groups I to IV, respectively. Collagen/Elastin ratio was 2.4; 5.1; 11.0 and 14.4 for groups I to IV, respectively. CONCLUSIONS: The biliary stenting leads to collagen and elastin deposition in the bile ducts; and collagen deposition and collagen/elastin ratio are proportional to the period of stenting.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2010
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oai:scielo:S0102-865020100002000062010-10-18Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometryArtifon,Everson Luiz de AlmeidaLopasso,Fabio PinatelMoura,Gustavo BrazunaFernandes,Fernando Augusto Mardiros HerbellaSakai,PauloCarrilho,Flair JoséCaldini,Elia Tamaso Espin GarciaSilva Junior,Orlando de Castro e Prostheses and Implants Collagen Elastin Common Bile Duct Rats PURPOSE: To evaluate the effects of biliary stenting in rats through analysis of collagen and elastin deposition in the bile ducts. METHODS: Twenty male rats underwent midline laparotomy, duodenotomy and transampullary stenting of the common bile duct with a 22Fr plastic stent. Animals were randomized in 4 groups, with 5 components in each: (I) control, (II) biliary stenting for 7 days, (III) biliary stenting for 14 days, and (IV) biliary stenting for 30 days. Sections of the common bile duct were stained using Hematoxylin Eosin; Sirius Red to quantitate the amount of collagen present; and Weigert's Resorcin-fuchsin to quantitate the amount of elastin present. RESULTS: The percentage of area stained for collagen was 13.4; 21.5; 29.5 and 32.8, for groups I to IV, respectively. The percentage of area stained for elastin fibers was 7.0; 5.2; 4.0 and 2.9, for groups I to IV, respectively. Collagen/Elastin ratio was 2.4; 5.1; 11.0 and 14.4 for groups I to IV, respectively. CONCLUSIONS: The biliary stenting leads to collagen and elastin deposition in the bile ducts; and collagen deposition and collagen/elastin ratio are proportional to the period of stenting.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.25 n.2 20102010-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200006en10.1590/S0102-86502010000200006 |
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Artifon,Everson Luiz de Almeida Lopasso,Fabio Pinatel Moura,Gustavo Brazuna Fernandes,Fernando Augusto Mardiros Herbella Sakai,Paulo Carrilho,Flair José Caldini,Elia Tamaso Espin Garcia Silva Junior,Orlando de Castro e |
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Artifon,Everson Luiz de Almeida Lopasso,Fabio Pinatel Moura,Gustavo Brazuna Fernandes,Fernando Augusto Mardiros Herbella Sakai,Paulo Carrilho,Flair José Caldini,Elia Tamaso Espin Garcia Silva Junior,Orlando de Castro e Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry |
author_facet |
Artifon,Everson Luiz de Almeida Lopasso,Fabio Pinatel Moura,Gustavo Brazuna Fernandes,Fernando Augusto Mardiros Herbella Sakai,Paulo Carrilho,Flair José Caldini,Elia Tamaso Espin Garcia Silva Junior,Orlando de Castro e |
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Artifon,Everson Luiz de Almeida |
title |
Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry |
title_short |
Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry |
title_full |
Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry |
title_fullStr |
Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry |
title_full_unstemmed |
Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry |
title_sort |
effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry |
description |
PURPOSE: To evaluate the effects of biliary stenting in rats through analysis of collagen and elastin deposition in the bile ducts. METHODS: Twenty male rats underwent midline laparotomy, duodenotomy and transampullary stenting of the common bile duct with a 22Fr plastic stent. Animals were randomized in 4 groups, with 5 components in each: (I) control, (II) biliary stenting for 7 days, (III) biliary stenting for 14 days, and (IV) biliary stenting for 30 days. Sections of the common bile duct were stained using Hematoxylin Eosin; Sirius Red to quantitate the amount of collagen present; and Weigert's Resorcin-fuchsin to quantitate the amount of elastin present. RESULTS: The percentage of area stained for collagen was 13.4; 21.5; 29.5 and 32.8, for groups I to IV, respectively. The percentage of area stained for elastin fibers was 7.0; 5.2; 4.0 and 2.9, for groups I to IV, respectively. Collagen/Elastin ratio was 2.4; 5.1; 11.0 and 14.4 for groups I to IV, respectively. CONCLUSIONS: The biliary stenting leads to collagen and elastin deposition in the bile ducts; and collagen deposition and collagen/elastin ratio are proportional to the period of stenting. |
publisher |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publishDate |
2010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200006 |
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