Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats

PURPOSE: Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. METHODS: Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. RESULTS: When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. CONCLUSION: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde.

Bibliographic Details

Main Authors:	Burlamaqui,Idália Maria Brasil, Dornelas,Conceição Aparecida, Escalante,Rodrigo Dornenfeld, Mota,Daniel Magalhães Coutinho, Mesquita,Francisco José Cabral, Carvalho,Eduardo Rebouças, Veras,Lara Burlamaqui, Rodrigues,Lusmar Veras
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2010
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200005
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