Evaluation of placental glycogen storage in mild diabetic rats
PURPOSE: To evaluate the placental glycogen storage and fetal development in the pregnancy of neonatally streptozocin-induced diabetic rats and to establish relation with glycemia and insulin levels. METHODS: At the birth day, 147 female rats were randomly distributed in two experimental groups: 1) Non-diabetic Group (Control, n=45) - received the vehicle; 2) Diabetic Group (STZ, n=102) - received 100 mg streptozocin/kg in neonatal period. At day 0 of pregnancy, adult female rats were included in the control group when presented glycemia below 120 mg/dL and, in the group STZ with glycemia between 120 and 300 mg/dL. At day 21 of pregnancy, blood samples were collected for glycemia and insulin determination, and placentas withdrawn for placental glycogen determination. The newborns (NB) were classified in small (SGA), appropriate (AGA) and large (LGA) for gestational age. RESULTS: Rats STZ presented higher glycemia at days 0 and 14 of pregnancy. At end of pregnancy, rats STZ showed higher proportion of NB SGA and LGA; reduced rate of NB AGA and unaltered glycemia, insulin and placental glycogen determinations. CONCLUSION: Mild diabetes altered the maternal glycemia in the early pregnancy, impairing future fetal development, but it caused no alteration on insulin and placental glycogen determination, confirming that this glycemic intensity was insufficient to change glycogen metabolism.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2010
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oai:scielo:S0102-865020100002000022010-10-18Evaluation of placental glycogen storage in mild diabetic ratsBueno,AlineIessi,Isabela LovizuttoCalderon,Iracema de Mattos ParanhosRudge,Marilza Vieira CunhaSantos,Carlos Eduardo Meirelles dosDamasceno,Débora Cristina Diabetes Mellitus Insulin Pregnancy, Animal Glycogen Streptozocin Glycemia Rats PURPOSE: To evaluate the placental glycogen storage and fetal development in the pregnancy of neonatally streptozocin-induced diabetic rats and to establish relation with glycemia and insulin levels. METHODS: At the birth day, 147 female rats were randomly distributed in two experimental groups: 1) Non-diabetic Group (Control, n=45) - received the vehicle; 2) Diabetic Group (STZ, n=102) - received 100 mg streptozocin/kg in neonatal period. At day 0 of pregnancy, adult female rats were included in the control group when presented glycemia below 120 mg/dL and, in the group STZ with glycemia between 120 and 300 mg/dL. At day 21 of pregnancy, blood samples were collected for glycemia and insulin determination, and placentas withdrawn for placental glycogen determination. The newborns (NB) were classified in small (SGA), appropriate (AGA) and large (LGA) for gestational age. RESULTS: Rats STZ presented higher glycemia at days 0 and 14 of pregnancy. At end of pregnancy, rats STZ showed higher proportion of NB SGA and LGA; reduced rate of NB AGA and unaltered glycemia, insulin and placental glycogen determinations. CONCLUSION: Mild diabetes altered the maternal glycemia in the early pregnancy, impairing future fetal development, but it caused no alteration on insulin and placental glycogen determination, confirming that this glycemic intensity was insufficient to change glycogen metabolism.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.25 n.2 20102010-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200002en10.1590/S0102-86502010000200002 |
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Bueno,Aline Iessi,Isabela Lovizutto Calderon,Iracema de Mattos Paranhos Rudge,Marilza Vieira Cunha Santos,Carlos Eduardo Meirelles dos Damasceno,Débora Cristina |
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Bueno,Aline Iessi,Isabela Lovizutto Calderon,Iracema de Mattos Paranhos Rudge,Marilza Vieira Cunha Santos,Carlos Eduardo Meirelles dos Damasceno,Débora Cristina Evaluation of placental glycogen storage in mild diabetic rats |
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Bueno,Aline Iessi,Isabela Lovizutto Calderon,Iracema de Mattos Paranhos Rudge,Marilza Vieira Cunha Santos,Carlos Eduardo Meirelles dos Damasceno,Débora Cristina |
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Bueno,Aline |
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Evaluation of placental glycogen storage in mild diabetic rats |
title_short |
Evaluation of placental glycogen storage in mild diabetic rats |
title_full |
Evaluation of placental glycogen storage in mild diabetic rats |
title_fullStr |
Evaluation of placental glycogen storage in mild diabetic rats |
title_full_unstemmed |
Evaluation of placental glycogen storage in mild diabetic rats |
title_sort |
evaluation of placental glycogen storage in mild diabetic rats |
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PURPOSE: To evaluate the placental glycogen storage and fetal development in the pregnancy of neonatally streptozocin-induced diabetic rats and to establish relation with glycemia and insulin levels. METHODS: At the birth day, 147 female rats were randomly distributed in two experimental groups: 1) Non-diabetic Group (Control, n=45) - received the vehicle; 2) Diabetic Group (STZ, n=102) - received 100 mg streptozocin/kg in neonatal period. At day 0 of pregnancy, adult female rats were included in the control group when presented glycemia below 120 mg/dL and, in the group STZ with glycemia between 120 and 300 mg/dL. At day 21 of pregnancy, blood samples were collected for glycemia and insulin determination, and placentas withdrawn for placental glycogen determination. The newborns (NB) were classified in small (SGA), appropriate (AGA) and large (LGA) for gestational age. RESULTS: Rats STZ presented higher glycemia at days 0 and 14 of pregnancy. At end of pregnancy, rats STZ showed higher proportion of NB SGA and LGA; reduced rate of NB AGA and unaltered glycemia, insulin and placental glycogen determinations. CONCLUSION: Mild diabetes altered the maternal glycemia in the early pregnancy, impairing future fetal development, but it caused no alteration on insulin and placental glycogen determination, confirming that this glycemic intensity was insufficient to change glycogen metabolism. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200002 |
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1756400869039931392 |