Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations

Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations

PURPOSE: This study sought to evaluate the efficiency of glycol methacrylate-embedding medium to detect morphological alterations of human saphenous vein submitted to brief and crescent pressurizations. METHODS: Saphenous veins of 20 CABG patients were randomly distributed into four experimental groups (control, 100, 200 and 300 mmHg pressures during 15 seconds). To quantify the percentage of endothelium spread over vein surface a microscope magnification of 100x was used for measurements. Morphometric analysis was performed using videomicroscopy with the Leica Qwin software in conjunction with a Leica microscope, videocamera, and an on-line computer. RESULTS: A slight tendency of quantitative increase was observed in all parameters including percentage of endothelium spread over vein surface and thickness of saphenous vein walls (intima and media layers). CONCLUSIONS: The glycol methacrylate-embedding allowed sections with adequate resolution of structural details and revealed to be an extremely useful method to study pressurized human saphenous veins.

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Bibliographic Details
Main Authors: Prado,Cibele M., Viaro,Fernanda, Baldo,Caroline F., Augusto,Viviane dos Santos, Rodrigues,Alfredo José, Evora,Paulo Roberto Barbosa
Format: Digital revista
Language:English
Published: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2008
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000700014
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spelling oai:scielo:S0102-865020080007000142008-05-20Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizationsPrado,Cibele M.Viaro,FernandaBaldo,Caroline F.Augusto,Viviane dos SantosRodrigues,Alfredo JoséEvora,Paulo Roberto Barbosa Saphenous Vein Endothelium Glycol Methacrylate-embedding Medium High Resolution Light Microscopy PURPOSE: This study sought to evaluate the efficiency of glycol methacrylate-embedding medium to detect morphological alterations of human saphenous vein submitted to brief and crescent pressurizations. METHODS: Saphenous veins of 20 CABG patients were randomly distributed into four experimental groups (control, 100, 200 and 300 mmHg pressures during 15 seconds). To quantify the percentage of endothelium spread over vein surface a microscope magnification of 100x was used for measurements. Morphometric analysis was performed using videomicroscopy with the Leica Qwin software in conjunction with a Leica microscope, videocamera, and an on-line computer. RESULTS: A slight tendency of quantitative increase was observed in all parameters including percentage of endothelium spread over vein surface and thickness of saphenous vein walls (intima and media layers). CONCLUSIONS: The glycol methacrylate-embedding allowed sections with adequate resolution of structural details and revealed to be an extremely useful method to study pressurized human saphenous veins.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.23 suppl.1 20082008-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000700014en10.1590/S0102-86502008000700014
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Prado,Cibele M.
Viaro,Fernanda
Baldo,Caroline F.
Augusto,Viviane dos Santos
Rodrigues,Alfredo José
Evora,Paulo Roberto Barbosa
spellingShingle Prado,Cibele M.
Viaro,Fernanda
Baldo,Caroline F.
Augusto,Viviane dos Santos
Rodrigues,Alfredo José
Evora,Paulo Roberto Barbosa
Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations
author_facet Prado,Cibele M.
Viaro,Fernanda
Baldo,Caroline F.
Augusto,Viviane dos Santos
Rodrigues,Alfredo José
Evora,Paulo Roberto Barbosa
author_sort Prado,Cibele M.
title Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations
title_short Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations
title_full Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations
title_fullStr Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations
title_full_unstemmed Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations
title_sort glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations
description PURPOSE: This study sought to evaluate the efficiency of glycol methacrylate-embedding medium to detect morphological alterations of human saphenous vein submitted to brief and crescent pressurizations. METHODS: Saphenous veins of 20 CABG patients were randomly distributed into four experimental groups (control, 100, 200 and 300 mmHg pressures during 15 seconds). To quantify the percentage of endothelium spread over vein surface a microscope magnification of 100x was used for measurements. Morphometric analysis was performed using videomicroscopy with the Leica Qwin software in conjunction with a Leica microscope, videocamera, and an on-line computer. RESULTS: A slight tendency of quantitative increase was observed in all parameters including percentage of endothelium spread over vein surface and thickness of saphenous vein walls (intima and media layers). CONCLUSIONS: The glycol methacrylate-embedding allowed sections with adequate resolution of structural details and revealed to be an extremely useful method to study pressurized human saphenous veins.
publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publishDate 2008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000700014
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