Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion
PURPOSE: To evaluate the influence of ischemia/reperfusion injury on renal compensatory growth (CGR) and mitochondrial function. METHODS: Forty five Wistar rats were divided in 3 groups: Control Group (GC) - 21 rats were submitted to a sham laparotomy and sacrificed at 1st (6 rats) and 7th (15 rats) postoperative days to evaluate the dry weight of both kidneys and their growth during 1 week (6 rats) and to quantify mitochondrial respiration (9 rats); Group 1 (G1) - 12 rats underwent right nephrectomy and were sacrificed 7 days later for analysis of renal mitochondrial function (6 rats) and dry weight (6 rats). Group 2 (G2) - renal warm ischemia for 60 minutes followed by right nephrectomy was performed in 12 rats; they were sacrificed 7 days later to evaluate renal mitochondrial function (6 rats) and dry weight (6 rats). RESULTS: Dry weight (mg) of left kidneys at 7th day: GC - 219±18, G1 - 281±23 and G2 - 338±39 (GCxG1 p<0.01; GCxG2 p<0.001; G1xG2 p<0.01). State 4 mitochondrial respiration rate and respiratory control ratio (RCR) were similar in all groups (p>0.05). State 3 respirations (mM/min/mg) in GC, G1 and G2 was respectively: 99±23, 132±22 and 82±44 (p<0.02; the only statistical difference noted was between groups G1xG2 - p<0.05). CONCLUSIONS: Following unilateral nephrectomy CRG is associated with an increase in state 3 of mitochondrial respiration. Renal ischemia/reperfusion injury enhances the CRG provoked by unilateral nephrectomy but such enhancement seems independent on mitochondrial respiration.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2008
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oai:scielo:S0102-865020080007000062008-05-20Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusionTucci Jr,SilvioMolina,Carlos Augusto FernandesCologna,Adauto JoséSuaid,Haylton JorgeTirapelli,Luis FernandoCelini,Fábia MartinsMartins,Antonio Carlos Pereira Ichemia Reperfusion Mitochondria Compensatory Renal Growth PURPOSE: To evaluate the influence of ischemia/reperfusion injury on renal compensatory growth (CGR) and mitochondrial function. METHODS: Forty five Wistar rats were divided in 3 groups: Control Group (GC) - 21 rats were submitted to a sham laparotomy and sacrificed at 1st (6 rats) and 7th (15 rats) postoperative days to evaluate the dry weight of both kidneys and their growth during 1 week (6 rats) and to quantify mitochondrial respiration (9 rats); Group 1 (G1) - 12 rats underwent right nephrectomy and were sacrificed 7 days later for analysis of renal mitochondrial function (6 rats) and dry weight (6 rats). Group 2 (G2) - renal warm ischemia for 60 minutes followed by right nephrectomy was performed in 12 rats; they were sacrificed 7 days later to evaluate renal mitochondrial function (6 rats) and dry weight (6 rats). RESULTS: Dry weight (mg) of left kidneys at 7th day: GC - 219±18, G1 - 281±23 and G2 - 338±39 (GCxG1 p<0.01; GCxG2 p<0.001; G1xG2 p<0.01). State 4 mitochondrial respiration rate and respiratory control ratio (RCR) were similar in all groups (p>0.05). State 3 respirations (mM/min/mg) in GC, G1 and G2 was respectively: 99±23, 132±22 and 82±44 (p<0.02; the only statistical difference noted was between groups G1xG2 - p<0.05). CONCLUSIONS: Following unilateral nephrectomy CRG is associated with an increase in state 3 of mitochondrial respiration. Renal ischemia/reperfusion injury enhances the CRG provoked by unilateral nephrectomy but such enhancement seems independent on mitochondrial respiration.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.23 suppl.1 20082008-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000700006en10.1590/S0102-86502008000700006 |
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Tucci Jr,Silvio Molina,Carlos Augusto Fernandes Cologna,Adauto José Suaid,Haylton Jorge Tirapelli,Luis Fernando Celini,Fábia Martins Martins,Antonio Carlos Pereira |
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Tucci Jr,Silvio Molina,Carlos Augusto Fernandes Cologna,Adauto José Suaid,Haylton Jorge Tirapelli,Luis Fernando Celini,Fábia Martins Martins,Antonio Carlos Pereira Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion |
author_facet |
Tucci Jr,Silvio Molina,Carlos Augusto Fernandes Cologna,Adauto José Suaid,Haylton Jorge Tirapelli,Luis Fernando Celini,Fábia Martins Martins,Antonio Carlos Pereira |
author_sort |
Tucci Jr,Silvio |
title |
Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion |
title_short |
Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion |
title_full |
Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion |
title_fullStr |
Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion |
title_full_unstemmed |
Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion |
title_sort |
compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion |
description |
PURPOSE: To evaluate the influence of ischemia/reperfusion injury on renal compensatory growth (CGR) and mitochondrial function. METHODS: Forty five Wistar rats were divided in 3 groups: Control Group (GC) - 21 rats were submitted to a sham laparotomy and sacrificed at 1st (6 rats) and 7th (15 rats) postoperative days to evaluate the dry weight of both kidneys and their growth during 1 week (6 rats) and to quantify mitochondrial respiration (9 rats); Group 1 (G1) - 12 rats underwent right nephrectomy and were sacrificed 7 days later for analysis of renal mitochondrial function (6 rats) and dry weight (6 rats). Group 2 (G2) - renal warm ischemia for 60 minutes followed by right nephrectomy was performed in 12 rats; they were sacrificed 7 days later to evaluate renal mitochondrial function (6 rats) and dry weight (6 rats). RESULTS: Dry weight (mg) of left kidneys at 7th day: GC - 219±18, G1 - 281±23 and G2 - 338±39 (GCxG1 p<0.01; GCxG2 p<0.001; G1xG2 p<0.01). State 4 mitochondrial respiration rate and respiratory control ratio (RCR) were similar in all groups (p>0.05). State 3 respirations (mM/min/mg) in GC, G1 and G2 was respectively: 99±23, 132±22 and 82±44 (p<0.02; the only statistical difference noted was between groups G1xG2 - p<0.05). CONCLUSIONS: Following unilateral nephrectomy CRG is associated with an increase in state 3 of mitochondrial respiration. Renal ischemia/reperfusion injury enhances the CRG provoked by unilateral nephrectomy but such enhancement seems independent on mitochondrial respiration. |
publisher |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publishDate |
2008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000700006 |
work_keys_str_mv |
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