Effects of simvastatin in abdominal sepsis in rats
PURPOSE: Statins are widely recognized as hypolipemic drugs, but some studies have observed anti-inflammatory and immunomodulatory effects, known as pleiotropic. The aims of this work was to study possible anti-inflammatory effects of simvastatin in abdominal sepsis. Serum pro-inflammatory cytokines and leukocytes count were determined in an experimental model of abdominal sepsis, using cecal ligation and puncture (CLP) in rats. METHODS: Twenty eigth Wistar rats weighing 285±12g were randomly divided in: CLP/Sinvastatin rats (n=7), treated with 10 mg/Kg of oral simvastatin 18 and 2 hs berofe CLP; CLP/Saline group rats (n=7), treated with oral saline; group Sham/Simvastatin (n=7), treated with simvastatin, and group Sham/Saline (n=7), treated with saline. Serum TNF-alpha, IL-1beta and IL-6 by ELISA and total leukocytes, neutrophils, lymphocytes, and eosinophils were determined 24 hs after CLP. ANOVA and Tukey test were used considering significant p<0.05. RESULTS: It was demonstrated that serum TNF-alpha, IL-1beta and IL-6 were respectively 364,8±42pg/mL; 46,3±18pg/mL and 28,4±13pg/mL in CLP/Sinvastatin rats, significantly lower (p<0.05) than in group CLP/Saline (778,5±86pg/ml; 176,9±46pg/ml; 133,6±21 pg/ml, respectively). The same results were observed in total leukocytes and neutrophils counts. CONCLUSION: These results clearly demonstrate that simvastatin is an effective agent that reduces cytokines levels and leukocyte count in sepsis, independently of its well-known lipid-lowering effects. Thus, HMG-CoA reductase inhibitors like simvastatin have important anti-inflammatory effects in abdominal sepsis in rats.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2006
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oai:scielo:S0102-865020060010000032007-02-07Effects of simvastatin in abdominal sepsis in ratsSouza Neto,José Luiz deAraújo Filho,IramiRego,Amália Cínthia Meneses doDominici,Víctor AlmeidaAzevedo,Ítalo MedeirosEgito,Eryvaldo Sócrates TabosaBrandão-Neto,JoséMedeiros,Aldo Cunha Statin Inflammation Abdominal sepsis Wistar rat Cytokine Leukocyte PURPOSE: Statins are widely recognized as hypolipemic drugs, but some studies have observed anti-inflammatory and immunomodulatory effects, known as pleiotropic. The aims of this work was to study possible anti-inflammatory effects of simvastatin in abdominal sepsis. Serum pro-inflammatory cytokines and leukocytes count were determined in an experimental model of abdominal sepsis, using cecal ligation and puncture (CLP) in rats. METHODS: Twenty eigth Wistar rats weighing 285±12g were randomly divided in: CLP/Sinvastatin rats (n=7), treated with 10 mg/Kg of oral simvastatin 18 and 2 hs berofe CLP; CLP/Saline group rats (n=7), treated with oral saline; group Sham/Simvastatin (n=7), treated with simvastatin, and group Sham/Saline (n=7), treated with saline. Serum TNF-alpha, IL-1beta and IL-6 by ELISA and total leukocytes, neutrophils, lymphocytes, and eosinophils were determined 24 hs after CLP. ANOVA and Tukey test were used considering significant p<0.05. RESULTS: It was demonstrated that serum TNF-alpha, IL-1beta and IL-6 were respectively 364,8±42pg/mL; 46,3±18pg/mL and 28,4±13pg/mL in CLP/Sinvastatin rats, significantly lower (p<0.05) than in group CLP/Saline (778,5±86pg/ml; 176,9±46pg/ml; 133,6±21 pg/ml, respectively). The same results were observed in total leukocytes and neutrophils counts. CONCLUSION: These results clearly demonstrate that simvastatin is an effective agent that reduces cytokines levels and leukocyte count in sepsis, independently of its well-known lipid-lowering effects. Thus, HMG-CoA reductase inhibitors like simvastatin have important anti-inflammatory effects in abdominal sepsis in rats.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.21 suppl.4 20062006-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006001000003en10.1590/S0102-86502006001000003 |
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Souza Neto,José Luiz de Araújo Filho,Irami Rego,Amália Cínthia Meneses do Dominici,Víctor Almeida Azevedo,Ítalo Medeiros Egito,Eryvaldo Sócrates Tabosa Brandão-Neto,José Medeiros,Aldo Cunha |
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Souza Neto,José Luiz de Araújo Filho,Irami Rego,Amália Cínthia Meneses do Dominici,Víctor Almeida Azevedo,Ítalo Medeiros Egito,Eryvaldo Sócrates Tabosa Brandão-Neto,José Medeiros,Aldo Cunha Effects of simvastatin in abdominal sepsis in rats |
author_facet |
Souza Neto,José Luiz de Araújo Filho,Irami Rego,Amália Cínthia Meneses do Dominici,Víctor Almeida Azevedo,Ítalo Medeiros Egito,Eryvaldo Sócrates Tabosa Brandão-Neto,José Medeiros,Aldo Cunha |
author_sort |
Souza Neto,José Luiz de |
title |
Effects of simvastatin in abdominal sepsis in rats |
title_short |
Effects of simvastatin in abdominal sepsis in rats |
title_full |
Effects of simvastatin in abdominal sepsis in rats |
title_fullStr |
Effects of simvastatin in abdominal sepsis in rats |
title_full_unstemmed |
Effects of simvastatin in abdominal sepsis in rats |
title_sort |
effects of simvastatin in abdominal sepsis in rats |
description |
PURPOSE: Statins are widely recognized as hypolipemic drugs, but some studies have observed anti-inflammatory and immunomodulatory effects, known as pleiotropic. The aims of this work was to study possible anti-inflammatory effects of simvastatin in abdominal sepsis. Serum pro-inflammatory cytokines and leukocytes count were determined in an experimental model of abdominal sepsis, using cecal ligation and puncture (CLP) in rats. METHODS: Twenty eigth Wistar rats weighing 285±12g were randomly divided in: CLP/Sinvastatin rats (n=7), treated with 10 mg/Kg of oral simvastatin 18 and 2 hs berofe CLP; CLP/Saline group rats (n=7), treated with oral saline; group Sham/Simvastatin (n=7), treated with simvastatin, and group Sham/Saline (n=7), treated with saline. Serum TNF-alpha, IL-1beta and IL-6 by ELISA and total leukocytes, neutrophils, lymphocytes, and eosinophils were determined 24 hs after CLP. ANOVA and Tukey test were used considering significant p<0.05. RESULTS: It was demonstrated that serum TNF-alpha, IL-1beta and IL-6 were respectively 364,8±42pg/mL; 46,3±18pg/mL and 28,4±13pg/mL in CLP/Sinvastatin rats, significantly lower (p<0.05) than in group CLP/Saline (778,5±86pg/ml; 176,9±46pg/ml; 133,6±21 pg/ml, respectively). The same results were observed in total leukocytes and neutrophils counts. CONCLUSION: These results clearly demonstrate that simvastatin is an effective agent that reduces cytokines levels and leukocyte count in sepsis, independently of its well-known lipid-lowering effects. Thus, HMG-CoA reductase inhibitors like simvastatin have important anti-inflammatory effects in abdominal sepsis in rats. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2006 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006001000003 |
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