A new method for the experimental induction of secundary biliary cirrhosis in wistar rats
The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2001
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oai:scielo:S0102-865020010002000032003-09-11A new method for the experimental induction of secundary biliary cirrhosis in wistar ratsJorge,Gracinda De LourdesLeonardi,Luiz SergioBoin,Ilka de Fatima Santana FerreiraSilva Jr,Orlando de Castro eEscanhoela,Cecilia Amelia Fazzio Secondary biliary cirrhosis Bile duct obstruction Cholestasis The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.16 n.2 20012001-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502001000200003en10.1590/S0102-86502001000200003 |
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Jorge,Gracinda De Lourdes Leonardi,Luiz Sergio Boin,Ilka de Fatima Santana Ferreira Silva Jr,Orlando de Castro e Escanhoela,Cecilia Amelia Fazzio |
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Jorge,Gracinda De Lourdes Leonardi,Luiz Sergio Boin,Ilka de Fatima Santana Ferreira Silva Jr,Orlando de Castro e Escanhoela,Cecilia Amelia Fazzio A new method for the experimental induction of secundary biliary cirrhosis in wistar rats |
author_facet |
Jorge,Gracinda De Lourdes Leonardi,Luiz Sergio Boin,Ilka de Fatima Santana Ferreira Silva Jr,Orlando de Castro e Escanhoela,Cecilia Amelia Fazzio |
author_sort |
Jorge,Gracinda De Lourdes |
title |
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats |
title_short |
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats |
title_full |
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats |
title_fullStr |
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats |
title_full_unstemmed |
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats |
title_sort |
new method for the experimental induction of secundary biliary cirrhosis in wistar rats |
description |
The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publishDate |
2001 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502001000200003 |
work_keys_str_mv |
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