Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury
Abstract Objective: The aim of the present study was to evaluate the ability of ischemic postconditioning, atorvastatin and both associated to prevent or minimize reperfusion injury in the lung of rats subjected to ischemia and reperfusion by abdominal aortic clamping. Methods: We used 41 Wistar norvegic rats, which were distributed into 5 groups: ischemia and reperfusion (I/R), ischemic postcondictioning (IPC), postconditioning + atorvastatin (IPC+A), atorvastatin (A) and SHAM. It was performed a medium laparotomy, dissection and isolation of the infra-renal abdominal aorta; except for the SHAM group, all the others were submitted to the aortic clamping for 70 minutes (ischemia) and posterior clamp removal (reperfusion, 70 minutes). In the IPC and IPC+A groups, postconditioning was performed between the ischemia and reperfusion phases by four cycles of reperfusion and ischemia lasting 30 seconds each. In the IPC+A and A groups, preceding the surgical procedure, administration of 3.4 mg/day of atorvastatin was performed for seven days by gavage. After the surgical procedure, the right caudal lobe was removed from the lung for histological study, using tissue injury score ranging from grade 1 (normal tissue) to grade 4 (intense lesion). Results: The mean lung injury was 3.6 in the I/R group, 1.6 in the IPC group, 1.2 in the IPC+A group, 1.2 in the A group, and 1 in the SHAM group (P<0.01). Conclusion: Ischemic postconditioning and atorvastatin were able to minimize lung reperfusion injury, alone or in combination.
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Sociedade Brasileira de Cirurgia Cardiovascular
2018
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oai:scielo:S0102-763820180002000022018-06-06Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion InjurySantos,Carlos Henrique Marques dosDourado,Doroty MesquitaSilva,Baldomero Antonio Kato daPontes,Henrique Budib DorsaAzevedo Neto,Euler deVendas,Giovanna Serra da CruzChaves,Ian de OliveiraMiranda,João Victor CunhaOliva,João Victor Durães GomesDias,Letícia do Espírito SantoAlmeida,Murillo Henrique Martins deSampaio,Trícia Luna Ischemia Reperfusion Injury Ischemic Postconditioning Hydroxymethylglutaryl-CoA Reductase Inhibitors Lung Abstract Objective: The aim of the present study was to evaluate the ability of ischemic postconditioning, atorvastatin and both associated to prevent or minimize reperfusion injury in the lung of rats subjected to ischemia and reperfusion by abdominal aortic clamping. Methods: We used 41 Wistar norvegic rats, which were distributed into 5 groups: ischemia and reperfusion (I/R), ischemic postcondictioning (IPC), postconditioning + atorvastatin (IPC+A), atorvastatin (A) and SHAM. It was performed a medium laparotomy, dissection and isolation of the infra-renal abdominal aorta; except for the SHAM group, all the others were submitted to the aortic clamping for 70 minutes (ischemia) and posterior clamp removal (reperfusion, 70 minutes). In the IPC and IPC+A groups, postconditioning was performed between the ischemia and reperfusion phases by four cycles of reperfusion and ischemia lasting 30 seconds each. In the IPC+A and A groups, preceding the surgical procedure, administration of 3.4 mg/day of atorvastatin was performed for seven days by gavage. After the surgical procedure, the right caudal lobe was removed from the lung for histological study, using tissue injury score ranging from grade 1 (normal tissue) to grade 4 (intense lesion). Results: The mean lung injury was 3.6 in the I/R group, 1.6 in the IPC group, 1.2 in the IPC+A group, 1.2 in the A group, and 1 in the SHAM group (P<0.01). Conclusion: Ischemic postconditioning and atorvastatin were able to minimize lung reperfusion injury, alone or in combination.info:eu-repo/semantics/openAccessSociedade Brasileira de Cirurgia CardiovascularBrazilian Journal of Cardiovascular Surgery v.33 n.2 20182018-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382018000200002en10.21470/1678-9741-2017-0022 |
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Santos,Carlos Henrique Marques dos Dourado,Doroty Mesquita Silva,Baldomero Antonio Kato da Pontes,Henrique Budib Dorsa Azevedo Neto,Euler de Vendas,Giovanna Serra da Cruz Chaves,Ian de Oliveira Miranda,João Victor Cunha Oliva,João Victor Durães Gomes Dias,Letícia do Espírito Santo Almeida,Murillo Henrique Martins de Sampaio,Trícia Luna |
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Santos,Carlos Henrique Marques dos Dourado,Doroty Mesquita Silva,Baldomero Antonio Kato da Pontes,Henrique Budib Dorsa Azevedo Neto,Euler de Vendas,Giovanna Serra da Cruz Chaves,Ian de Oliveira Miranda,João Victor Cunha Oliva,João Victor Durães Gomes Dias,Letícia do Espírito Santo Almeida,Murillo Henrique Martins de Sampaio,Trícia Luna Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury |
author_facet |
Santos,Carlos Henrique Marques dos Dourado,Doroty Mesquita Silva,Baldomero Antonio Kato da Pontes,Henrique Budib Dorsa Azevedo Neto,Euler de Vendas,Giovanna Serra da Cruz Chaves,Ian de Oliveira Miranda,João Victor Cunha Oliva,João Victor Durães Gomes Dias,Letícia do Espírito Santo Almeida,Murillo Henrique Martins de Sampaio,Trícia Luna |
author_sort |
Santos,Carlos Henrique Marques dos |
title |
Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury |
title_short |
Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury |
title_full |
Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury |
title_fullStr |
Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury |
title_full_unstemmed |
Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury |
title_sort |
effect of ischemic postconditioning and atorvastatin in the prevention of remote lung reperfusion injury |
description |
Abstract Objective: The aim of the present study was to evaluate the ability of ischemic postconditioning, atorvastatin and both associated to prevent or minimize reperfusion injury in the lung of rats subjected to ischemia and reperfusion by abdominal aortic clamping. Methods: We used 41 Wistar norvegic rats, which were distributed into 5 groups: ischemia and reperfusion (I/R), ischemic postcondictioning (IPC), postconditioning + atorvastatin (IPC+A), atorvastatin (A) and SHAM. It was performed a medium laparotomy, dissection and isolation of the infra-renal abdominal aorta; except for the SHAM group, all the others were submitted to the aortic clamping for 70 minutes (ischemia) and posterior clamp removal (reperfusion, 70 minutes). In the IPC and IPC+A groups, postconditioning was performed between the ischemia and reperfusion phases by four cycles of reperfusion and ischemia lasting 30 seconds each. In the IPC+A and A groups, preceding the surgical procedure, administration of 3.4 mg/day of atorvastatin was performed for seven days by gavage. After the surgical procedure, the right caudal lobe was removed from the lung for histological study, using tissue injury score ranging from grade 1 (normal tissue) to grade 4 (intense lesion). Results: The mean lung injury was 3.6 in the I/R group, 1.6 in the IPC group, 1.2 in the IPC+A group, 1.2 in the A group, and 1 in the SHAM group (P<0.01). Conclusion: Ischemic postconditioning and atorvastatin were able to minimize lung reperfusion injury, alone or in combination. |
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Sociedade Brasileira de Cirurgia Cardiovascular |
publishDate |
2018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382018000200002 |
work_keys_str_mv |
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