Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis

Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis

AbstractIntroduction:Rheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A.Objective:The aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves.Methods:Immunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples.Results:ET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis.Conclusion:The strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.

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Main Authors: Leão,Sydney Correia, Dashwood,Michael R., Andrade,Mateus Santana de, Santos,Nicolas Nascimento, Teles,Olivia Regina Lins Leal, Souza,Williasmin Batista de, Rodrigues,Tania Maria de Andrade
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Cirurgia Cardiovascular 2015
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200012
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spelling oai:scielo:S0102-763820150002000122015-10-09Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosisLeão,Sydney CorreiaDashwood,Michael R.Andrade,Mateus Santana deSantos,Nicolas NascimentoTeles,Olivia Regina Lins LealSouza,Williasmin Batista deRodrigues,Tania Maria de Andrade Rheumatic Fever Mitral Valve Stenosis Endothelin- 1 Immunohistochemistry Blotting, Western AbstractIntroduction:Rheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A.Objective:The aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves.Methods:Immunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples.Results:ET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis.Conclusion:The strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.info:eu-repo/semantics/openAccessSociedade Brasileira de Cirurgia CardiovascularBrazilian Journal of Cardiovascular Surgery v.30 n.2 20152015-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200012en10.5935/1678-9741.20150004
institution SCIELO
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country Brasil
countrycode BR
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Leão,Sydney Correia
Dashwood,Michael R.
Andrade,Mateus Santana de
Santos,Nicolas Nascimento
Teles,Olivia Regina Lins Leal
Souza,Williasmin Batista de
Rodrigues,Tania Maria de Andrade
spellingShingle Leão,Sydney Correia
Dashwood,Michael R.
Andrade,Mateus Santana de
Santos,Nicolas Nascimento
Teles,Olivia Regina Lins Leal
Souza,Williasmin Batista de
Rodrigues,Tania Maria de Andrade
Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
author_facet Leão,Sydney Correia
Dashwood,Michael R.
Andrade,Mateus Santana de
Santos,Nicolas Nascimento
Teles,Olivia Regina Lins Leal
Souza,Williasmin Batista de
Rodrigues,Tania Maria de Andrade
author_sort Leão,Sydney Correia
title Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
title_short Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
title_full Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
title_fullStr Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
title_full_unstemmed Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
title_sort analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
description AbstractIntroduction:Rheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A.Objective:The aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves.Methods:Immunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples.Results:ET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis.Conclusion:The strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.
publisher Sociedade Brasileira de Cirurgia Cardiovascular
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200012
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