Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans

Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans

Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.

Saved in:
Bibliographic Details
Main Authors: Ducatti,Diogo R. B., Colodi,Franciely G., Gonçalves,Alan G., Duarte,M. Eugênia R., Noseda,Miguel D.
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Farmacognosia 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200015
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0102-695X2011000200015
record_format ojs
spelling oai:scielo:S0102-695X20110002000152011-06-03Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactansDucatti,Diogo R. B.Colodi,Franciely G.Gonçalves,Alan G.Duarte,M. Eugênia R.Noseda,Miguel D. agaro-oligosaccharides carra-oligosaccharides partial acid hydrolysis 3,6-anhydro-galactose agarose kappa-carrageenan Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.info:eu-repo/semantics/openAccessSociedade Brasileira de FarmacognosiaRevista Brasileira de Farmacognosia v.21 n.2 20112011-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200015en10.1590/S0102-695X2011005000080
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Ducatti,Diogo R. B.
Colodi,Franciely G.
Gonçalves,Alan G.
Duarte,M. Eugênia R.
Noseda,Miguel D.
spellingShingle Ducatti,Diogo R. B.
Colodi,Franciely G.
Gonçalves,Alan G.
Duarte,M. Eugênia R.
Noseda,Miguel D.
Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
author_facet Ducatti,Diogo R. B.
Colodi,Franciely G.
Gonçalves,Alan G.
Duarte,M. Eugênia R.
Noseda,Miguel D.
author_sort Ducatti,Diogo R. B.
title Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_short Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_full Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_fullStr Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_full_unstemmed Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_sort production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
description Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.
publisher Sociedade Brasileira de Farmacognosia
publishDate 2011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200015
work_keys_str_mv AT ducattidiogorb productionofagaroandcarraoligosaccharidesbypartialacidhydrolysisofgalactans
AT colodifrancielyg productionofagaroandcarraoligosaccharidesbypartialacidhydrolysisofgalactans
AT goncalvesalang productionofagaroandcarraoligosaccharidesbypartialacidhydrolysisofgalactans
AT duartemeugeniar productionofagaroandcarraoligosaccharidesbypartialacidhydrolysisofgalactans
AT nosedamigueld productionofagaroandcarraoligosaccharidesbypartialacidhydrolysisofgalactans
_version_ 1756399341943128064

Similar Items