Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves
In this work, chemical and biological activities of crude extracts obtained with 50% ethanol, 70% ethanol, acetone:water (7:3; v/v) and chloroform of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves, a native tree from several regions of Brazil, was investigated. Histochemical and phytochemical screenings were done according to characterization reactions and thin layer chromatography. To assist in extracts standardization, total phenol and flavonoids content spectrophotometric was performed. Antioxidant activity was analyzed by percentage of radical scavenging using DPPH solution. Antimicrobial activity was evaluated against Gram-positive, Gram-negative pathogenic bacteria and species of Candida using agar diffusion and minimal inhibitory concentration (MIC) determination methods according to standard methods. The leaves presented lipids at secretory cavity and phenols, mainly tannins, in nervures and palisade parenchyma. Polar extracts showed flavonoids, tannins and high content of phenols and flavonoids. The extracts showed great antioxidant activity and antimicrobial activity was better against Candida species than against bacteria.
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Sociedade Brasileira de Farmacognosia
2010
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oai:scielo:S0102-695X20100001000112010-04-26Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leavesSouza-Moreira,Tatiana M.Moreira,Raquel R. D.Sacramento,Luis V. S.Pietro,Rosemeire C. L. R. Plinia cauliflora chemical screening antioxidant activity antimicrobial activity In this work, chemical and biological activities of crude extracts obtained with 50% ethanol, 70% ethanol, acetone:water (7:3; v/v) and chloroform of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves, a native tree from several regions of Brazil, was investigated. Histochemical and phytochemical screenings were done according to characterization reactions and thin layer chromatography. To assist in extracts standardization, total phenol and flavonoids content spectrophotometric was performed. Antioxidant activity was analyzed by percentage of radical scavenging using DPPH solution. Antimicrobial activity was evaluated against Gram-positive, Gram-negative pathogenic bacteria and species of Candida using agar diffusion and minimal inhibitory concentration (MIC) determination methods according to standard methods. The leaves presented lipids at secretory cavity and phenols, mainly tannins, in nervures and palisade parenchyma. Polar extracts showed flavonoids, tannins and high content of phenols and flavonoids. The extracts showed great antioxidant activity and antimicrobial activity was better against Candida species than against bacteria.info:eu-repo/semantics/openAccessSociedade Brasileira de FarmacognosiaRevista Brasileira de Farmacognosia v.20 n.1 20102010-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2010000100011en10.1590/S0102-695X2010000100011 |
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Souza-Moreira,Tatiana M. Moreira,Raquel R. D. Sacramento,Luis V. S. Pietro,Rosemeire C. L. R. |
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Souza-Moreira,Tatiana M. Moreira,Raquel R. D. Sacramento,Luis V. S. Pietro,Rosemeire C. L. R. Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves |
author_facet |
Souza-Moreira,Tatiana M. Moreira,Raquel R. D. Sacramento,Luis V. S. Pietro,Rosemeire C. L. R. |
author_sort |
Souza-Moreira,Tatiana M. |
title |
Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves |
title_short |
Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves |
title_full |
Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves |
title_fullStr |
Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves |
title_full_unstemmed |
Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves |
title_sort |
histochemical, phytochemical and biological screening of plinia cauliflora (dc.) kausel, myrtaceae, leaves |
description |
In this work, chemical and biological activities of crude extracts obtained with 50% ethanol, 70% ethanol, acetone:water (7:3; v/v) and chloroform of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves, a native tree from several regions of Brazil, was investigated. Histochemical and phytochemical screenings were done according to characterization reactions and thin layer chromatography. To assist in extracts standardization, total phenol and flavonoids content spectrophotometric was performed. Antioxidant activity was analyzed by percentage of radical scavenging using DPPH solution. Antimicrobial activity was evaluated against Gram-positive, Gram-negative pathogenic bacteria and species of Candida using agar diffusion and minimal inhibitory concentration (MIC) determination methods according to standard methods. The leaves presented lipids at secretory cavity and phenols, mainly tannins, in nervures and palisade parenchyma. Polar extracts showed flavonoids, tannins and high content of phenols and flavonoids. The extracts showed great antioxidant activity and antimicrobial activity was better against Candida species than against bacteria. |
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Sociedade Brasileira de Farmacognosia |
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2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2010000100011 |
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1756399282191073280 |