Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production

Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production

ABSTRACT Ustilago maydis is a basidiomycete known as the causative agent of 'common smut', worldwide disease of maize that is recognized by the galls it forms, which have considerable potential as a gourmet food. Results of infection are quite variable, even under optimal greenhouse conditions. In order to find pathogenic strains able to be used as a highly infective and stable inoculum for the successful production of galls either in greenhouses or in the field, ears with gall symptoms containing teliospores were recovered from maize plants. The teliospores were suspended in water and plated on nutrient-rich medium. Twenty-six colonies developed, containing three types of yeast-like colonies: saprotrophic, pathogenic, and solopathogenic. DAPI staining confirmed the presence of solopathogenic strains with diploid sporidia. Groups of different mating types were found when pairs of the 26 strains were arranged resembling partial-diallel combinations. Amplification of the partial b locus revealed that the strains found harbor the alleles b3 and b4, allowing the formation in dikaryotic strains of heterodimeric regulatory proteins associated with fungal development and pathogenicity. In this study, we isolated compatible haploid and solopathogenic diploid strains for their high capacity for inducing smut.

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Main Authors: Galicia-García,Porfirio Raúl, Silva-Rojas,Hilda Victoria, Mendoza-Onofre,Leopoldo Ernesto, Zavaleta-Mancera,Hilda Araceli, Córdova-Téllez,Leobigildo, Espinosa-Calderón,Alejandro
Format: Digital revista
Language:English
Published: Sociedade Botânica do Brasil 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000400683
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spelling oai:scielo:S0102-330620160004006832017-01-11Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche productionGalicia-García,Porfirio RaúlSilva-Rojas,Hilda VictoriaMendoza-Onofre,Leopoldo ErnestoZavaleta-Mancera,Hilda AraceliCórdova-Téllez,LeobigildoEspinosa-Calderón,Alejandro Basidiomycete maize galls mating types molecular diagnosis smut ABSTRACT Ustilago maydis is a basidiomycete known as the causative agent of 'common smut', worldwide disease of maize that is recognized by the galls it forms, which have considerable potential as a gourmet food. Results of infection are quite variable, even under optimal greenhouse conditions. In order to find pathogenic strains able to be used as a highly infective and stable inoculum for the successful production of galls either in greenhouses or in the field, ears with gall symptoms containing teliospores were recovered from maize plants. The teliospores were suspended in water and plated on nutrient-rich medium. Twenty-six colonies developed, containing three types of yeast-like colonies: saprotrophic, pathogenic, and solopathogenic. DAPI staining confirmed the presence of solopathogenic strains with diploid sporidia. Groups of different mating types were found when pairs of the 26 strains were arranged resembling partial-diallel combinations. Amplification of the partial b locus revealed that the strains found harbor the alleles b3 and b4, allowing the formation in dikaryotic strains of heterodimeric regulatory proteins associated with fungal development and pathogenicity. In this study, we isolated compatible haploid and solopathogenic diploid strains for their high capacity for inducing smut.info:eu-repo/semantics/openAccessSociedade Botânica do BrasilActa Botanica Brasilica v.30 n.4 20162016-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000400683en10.1590/0102-33062016abb0097
institution SCIELO
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country Brasil
countrycode BR
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access En linea
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region America del Sur
libraryname SciELO
language English
format Digital
author Galicia-García,Porfirio Raúl
Silva-Rojas,Hilda Victoria
Mendoza-Onofre,Leopoldo Ernesto
Zavaleta-Mancera,Hilda Araceli
Córdova-Téllez,Leobigildo
Espinosa-Calderón,Alejandro
spellingShingle Galicia-García,Porfirio Raúl
Silva-Rojas,Hilda Victoria
Mendoza-Onofre,Leopoldo Ernesto
Zavaleta-Mancera,Hilda Araceli
Córdova-Téllez,Leobigildo
Espinosa-Calderón,Alejandro
Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production
author_facet Galicia-García,Porfirio Raúl
Silva-Rojas,Hilda Victoria
Mendoza-Onofre,Leopoldo Ernesto
Zavaleta-Mancera,Hilda Araceli
Córdova-Téllez,Leobigildo
Espinosa-Calderón,Alejandro
author_sort Galicia-García,Porfirio Raúl
title Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production
title_short Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production
title_full Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production
title_fullStr Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production
title_full_unstemmed Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production
title_sort selection of aggressive pathogenic and solopathogenic strains of ustilago maydis to improve huitlacoche production
description ABSTRACT Ustilago maydis is a basidiomycete known as the causative agent of 'common smut', worldwide disease of maize that is recognized by the galls it forms, which have considerable potential as a gourmet food. Results of infection are quite variable, even under optimal greenhouse conditions. In order to find pathogenic strains able to be used as a highly infective and stable inoculum for the successful production of galls either in greenhouses or in the field, ears with gall symptoms containing teliospores were recovered from maize plants. The teliospores were suspended in water and plated on nutrient-rich medium. Twenty-six colonies developed, containing three types of yeast-like colonies: saprotrophic, pathogenic, and solopathogenic. DAPI staining confirmed the presence of solopathogenic strains with diploid sporidia. Groups of different mating types were found when pairs of the 26 strains were arranged resembling partial-diallel combinations. Amplification of the partial b locus revealed that the strains found harbor the alleles b3 and b4, allowing the formation in dikaryotic strains of heterodimeric regulatory proteins associated with fungal development and pathogenicity. In this study, we isolated compatible haploid and solopathogenic diploid strains for their high capacity for inducing smut.
publisher Sociedade Botânica do Brasil
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000400683
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