Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

ABSTRACT The effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P>0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.

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Main Authors: Pradieé,J., Cardoso,T.F., Silva,E.F., Gonçalves,A.O., Gastal,G.D.A., Rosa,C.E., Mondadori,R.G., Pegoraro,L.M.C., Vieira,A.D., Lucia Jr.,T.
Format: Digital revista
Language:English
Published: Universidade Federal de Minas Gerais, Escola de Veterinária 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501309
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spelling oai:scielo:S0102-093520160005013092016-11-07Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryosPradieé,J.Cardoso,T.F.Silva,E.F.Gonçalves,A.O.Gastal,G.D.A.Rosa,C.E.Mondadori,R.G.Pegoraro,L.M.C.Vieira,A.D.Lucia Jr.,T. sheep embryos antioxidant reactive oxygen species frozen ram sperm vitrification ABSTRACT The effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P>0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.info:eu-repo/semantics/openAccessUniversidade Federal de Minas Gerais, Escola de VeterináriaArquivo Brasileiro de Medicina Veterinária e Zootecnia v.68 n.5 20162016-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501309en10.1590/1678-4162-8479
institution SCIELO
collection OJS
country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Pradieé,J.
Cardoso,T.F.
Silva,E.F.
Gonçalves,A.O.
Gastal,G.D.A.
Rosa,C.E.
Mondadori,R.G.
Pegoraro,L.M.C.
Vieira,A.D.
Lucia Jr.,T.
spellingShingle Pradieé,J.
Cardoso,T.F.
Silva,E.F.
Gonçalves,A.O.
Gastal,G.D.A.
Rosa,C.E.
Mondadori,R.G.
Pegoraro,L.M.C.
Vieira,A.D.
Lucia Jr.,T.
Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos
author_facet Pradieé,J.
Cardoso,T.F.
Silva,E.F.
Gonçalves,A.O.
Gastal,G.D.A.
Rosa,C.E.
Mondadori,R.G.
Pegoraro,L.M.C.
Vieira,A.D.
Lucia Jr.,T.
author_sort Pradieé,J.
title Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos
title_short Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos
title_full Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos
title_fullStr Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos
title_full_unstemmed Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos
title_sort effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos
description ABSTRACT The effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P>0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.
publisher Universidade Federal de Minas Gerais, Escola de Veterinária
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501309
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