Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs

The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.

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Main Authors: Figueiredo,F.C., Lopes,P.S., Pinto,A.P.G., Paiva,D.A.F., Mendonça,P.T., Guimarães,S.E.F.
Format: Digital revista
Language:English
Published: Universidade Federal de Minas Gerais, Escola de Veterinária 2008
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200020
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spelling oai:scielo:S0102-093520080002000202008-06-05Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigsFigueiredo,F.C.Lopes,P.S.Pinto,A.P.G.Paiva,D.A.F.Mendonça,P.T.Guimarães,S.E.F. swine candidate gene molecular marker DNA polymorphism DNA sequencing The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.info:eu-repo/semantics/openAccessUniversidade Federal de Minas Gerais, Escola de VeterináriaArquivo Brasileiro de Medicina Veterinária e Zootecnia v.60 n.2 20082008-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200020en10.1590/S0102-09352008000200020
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Figueiredo,F.C.
Lopes,P.S.
Pinto,A.P.G.
Paiva,D.A.F.
Mendonça,P.T.
Guimarães,S.E.F.
spellingShingle Figueiredo,F.C.
Lopes,P.S.
Pinto,A.P.G.
Paiva,D.A.F.
Mendonça,P.T.
Guimarães,S.E.F.
Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
author_facet Figueiredo,F.C.
Lopes,P.S.
Pinto,A.P.G.
Paiva,D.A.F.
Mendonça,P.T.
Guimarães,S.E.F.
author_sort Figueiredo,F.C.
title Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
title_short Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
title_full Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
title_fullStr Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
title_full_unstemmed Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
title_sort association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
description The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.
publisher Universidade Federal de Minas Gerais, Escola de Veterinária
publishDate 2008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200020
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