Efficiency of methods to detect Sclerotinia sclerotiorum in commercial soybean seed lots
Most soybean pathogens are seed transmitted, deserving emphasis the fungus Sclerotinia sclerotiorum, which has been presenting worrying levels of field incidence in some soybean cropping areas in several Brazilian states. The objective of this study was to verify the efficiency of different methods for detecting S. sclerotiorum on soybean seeds artificially infected in the laboratory and from field production areas with a historical disease incidence. Seed samples of seven different cultivars collected from naturally infested fields, and one seed sample artificially inoculated in the laboratory were used. The following detection methods recommended in the literature were compared: Blotter test at 7 ºC, 14 ºC, and 21 ºC; Rolled Paper; and Neon-S. Results demonstrated that these methods showed no repeatability and had a low sensitivity for detecting the pathogen in seeds from areas with disease incidence. They were effective, however, for its detection on artificially inoculated seeds. In the Blotter test method at 7 ºC, there was a lower incidence of other fungi considered undesirable during seed analysis.
Main Authors: | Henneberg,Luciane, Jaccoud Filho,David de S., Ruaro,Lucimeris, Panobianco,Maristela |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Associação Brasileira de Tecnologia de Sementes
2012
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000100008 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds
by: Grabicoski,Edilaine Mauricia Gelinski, et al.
Published: (2015) -
Incidência de Sclerotinia sclerotiorum em sementes de soja e sensibilidade dos testes de detecção
by: Henneberg,Luciane, et al.
Published: (2012) -
Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques
by: Botelho,Luana da Silva, et al.
Published: (2015) -
Establishment of a semi-selective method for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds
by: Kawasaki,Vivian Hikari, et al.
Published: (2013) -
Use of semi-seletive media for detection of Sclerotinia sclerotiorum on bean and soybean seeds
by: PERES,ÂNGELA P., et al.
Published: (2002)