Identification and molecular docking of two novel peptides with xanthine oxidase inhibitory activity from Auxis thazard
Abstract Auxis thazard meat was hydrolyzed by alkaline protease. Auxis thazard hydrolysate (ATH) obtained was isolated by ultrafiltration, size exclusion chromatography and reversed-phase high-performance liquid chromatography. Two peptides with high XOD inhibitory activity purified from ATH were identified as Pro-Asp-Leu (PDL, 344.87 Da) and Ser-Val-Gly-Gly-Ala-Leu (SVGGAL, 504.26 Da) by UPLC-MS/MS, which possessed high in vitro XOD inhibitory activity with the IC50 values of 4.37 ± 0.11 mg mL-1 and 5.59 ± 0.09 mg mL-1, respectively. Molecular simulation indicated that PDL and SVGGAL binded to XOD mainly through hydrogen bond and hydrophobic interaction, thereby inhibiting XOD activity. The research results suggested that the two peptides had potential application prospects as a safe XOD inhibitor substance for hyperuricemia treatment.
Main Authors: | WEI,Liuyi, JI,Hongwu, SONG,Wenkui, PENG,Shuo, ZHAN,Suhong, QU,Yushan, CHEN,Ming, ZHANG,Di, LIU,Shucheng |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2022
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100915 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Anti-fatigue effect of small molecule oligopeptides from tilapia (Oreochromis Mossambicus) in mice
by: PENG,Shuo, et al.
Published: (2022) -
The calcium supplementation effect of calcium-binding oligopeptides from bonito (Auxis thazard) hydrolysate in rats
by: JI,Wei, et al.
Published: (2022) -
Extraction optimization of polyphenols, antioxidant and xanthine oxidase inhibitory activities from Prunus salicina Lindl.
by: LI,Yibin, et al.
Published: (2016) -
Frigate tuna (Auxis thazard) fishery in Maldives
Published: (2013) -
Status of the Indian Ocean frigate tuna (FRI: Auxis thazard) resource
Published: (2013)