Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2017
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oai:scielo:S0101-206120170001001032017-02-21Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheatFARIA,Carla BertechiniSANTOS,Fabiane Cristina dosCASTRO,Fausto Fernandes deSUTIL,Ariadne RicieliSERGIO,Luciana MarcianoSILVA,Milena VeroneziMACHINSKI JUNIOR,MiguelBARBOSA-TESSMANN,Ione Parra Aspergillus flavus aflatoxins Bulgur wheat PCR Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.info:eu-repo/semantics/openAccessSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology v.37 n.1 20172017-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103en10.1590/1678-457x.09316 |
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FARIA,Carla Bertechini SANTOS,Fabiane Cristina dos CASTRO,Fausto Fernandes de SUTIL,Ariadne Ricieli SERGIO,Luciana Marciano SILVA,Milena Veronezi MACHINSKI JUNIOR,Miguel BARBOSA-TESSMANN,Ione Parra |
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FARIA,Carla Bertechini SANTOS,Fabiane Cristina dos CASTRO,Fausto Fernandes de SUTIL,Ariadne Ricieli SERGIO,Luciana Marciano SILVA,Milena Veronezi MACHINSKI JUNIOR,Miguel BARBOSA-TESSMANN,Ione Parra Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat |
author_facet |
FARIA,Carla Bertechini SANTOS,Fabiane Cristina dos CASTRO,Fausto Fernandes de SUTIL,Ariadne Ricieli SERGIO,Luciana Marciano SILVA,Milena Veronezi MACHINSKI JUNIOR,Miguel BARBOSA-TESSMANN,Ione Parra |
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FARIA,Carla Bertechini |
title |
Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat |
title_short |
Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat |
title_full |
Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat |
title_fullStr |
Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat |
title_full_unstemmed |
Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat |
title_sort |
occurrence of toxigenic aspergillus flavus in commercial bulgur wheat |
description |
Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning. |
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
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2017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103 |
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