Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat

Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat

Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.

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Main Authors: FARIA,Carla Bertechini, SANTOS,Fabiane Cristina dos, CASTRO,Fausto Fernandes de, SUTIL,Ariadne Ricieli, SERGIO,Luciana Marciano, SILVA,Milena Veronezi, MACHINSKI JUNIOR,Miguel, BARBOSA-TESSMANN,Ione Parra
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Ciência e Tecnologia de Alimentos 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103
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spelling oai:scielo:S0101-206120170001001032017-02-21Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheatFARIA,Carla BertechiniSANTOS,Fabiane Cristina dosCASTRO,Fausto Fernandes deSUTIL,Ariadne RicieliSERGIO,Luciana MarcianoSILVA,Milena VeroneziMACHINSKI JUNIOR,MiguelBARBOSA-TESSMANN,Ione Parra Aspergillus flavus aflatoxins Bulgur wheat PCR Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.info:eu-repo/semantics/openAccessSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology v.37 n.1 20172017-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103en10.1590/1678-457x.09316
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libraryname SciELO
language English
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author FARIA,Carla Bertechini
SANTOS,Fabiane Cristina dos
CASTRO,Fausto Fernandes de
SUTIL,Ariadne Ricieli
SERGIO,Luciana Marciano
SILVA,Milena Veronezi
MACHINSKI JUNIOR,Miguel
BARBOSA-TESSMANN,Ione Parra
spellingShingle FARIA,Carla Bertechini
SANTOS,Fabiane Cristina dos
CASTRO,Fausto Fernandes de
SUTIL,Ariadne Ricieli
SERGIO,Luciana Marciano
SILVA,Milena Veronezi
MACHINSKI JUNIOR,Miguel
BARBOSA-TESSMANN,Ione Parra
Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
author_facet FARIA,Carla Bertechini
SANTOS,Fabiane Cristina dos
CASTRO,Fausto Fernandes de
SUTIL,Ariadne Ricieli
SERGIO,Luciana Marciano
SILVA,Milena Veronezi
MACHINSKI JUNIOR,Miguel
BARBOSA-TESSMANN,Ione Parra
author_sort FARIA,Carla Bertechini
title Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_short Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_full Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_fullStr Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_full_unstemmed Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat
title_sort occurrence of toxigenic aspergillus flavus in commercial bulgur wheat
description Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.
publisher Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103
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