Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia

Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia

Autophagy is a lysosomal degradation pathway that removes protein aggregates and damaged organelles maintaining cellular integrity. It seems to be essential for cell survival during stress, starvation, hypoxia, and consequently to the placenta implantation and development. Preeclampsia (PE) is a multisystemic disorder characterized by the onset of hypertension associated or not with proteinuria and other maternal complications. Considering that the placenta seems to play an important role in the pathogenesis of PE, the objective of the present study was to evaluate protein levels of light chain protein (LC3), beclin-1, and the mammalian target of rapamycin (mTOR) in the placenta of pregnant women with PE. Placental tissues collected from 20 women with PE and 20 normotensive (NT) pregnant women were evaluated for LC3, beclin-1, and mTOR expression by qPCR and immunohistochemistry. The mRNA for LC3 and beclin-1 were significantly lower, while mTOR gene expression was significantly higher in the placenta of pregnant women with PE than in the NT group. Placentas of PE women showed significantly decreased protein expression of LC3-II and beclin-1, whereas mTOR was significantly increased compared with the NT pregnant women. There was a negative correlation between protein expression of mTOR and LC3-II in the placental tissue of PE women. In conclusion, the results showed autophagy deficiency suggesting that failure in this degradation process may contribute to the pathogenesis of PE; however, new studies involving cross-talk between autophagy and inflammatory molecular mechanisms might help to better understand the autophagy process in this obstetric pathology.

Saved in:
Bibliographic Details
Main Authors: Weel,I.C., Ribeiro,V.R., Romão-Veiga,M., Fioratti,E.G., Peraçoli,J.C., Peraçoli,M.T.S.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100683
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0100-879X2022000100683
record_format ojs
spelling oai:scielo:S0100-879X20220001006832023-01-04Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsiaWeel,I.C.Ribeiro,V.R.Romão-Veiga,M.Fioratti,E.G.Peraçoli,J.C.Peraçoli,M.T.S. Autophagy LC3 Beclin-1 mTOR Placenta Preeclampsia Autophagy is a lysosomal degradation pathway that removes protein aggregates and damaged organelles maintaining cellular integrity. It seems to be essential for cell survival during stress, starvation, hypoxia, and consequently to the placenta implantation and development. Preeclampsia (PE) is a multisystemic disorder characterized by the onset of hypertension associated or not with proteinuria and other maternal complications. Considering that the placenta seems to play an important role in the pathogenesis of PE, the objective of the present study was to evaluate protein levels of light chain protein (LC3), beclin-1, and the mammalian target of rapamycin (mTOR) in the placenta of pregnant women with PE. Placental tissues collected from 20 women with PE and 20 normotensive (NT) pregnant women were evaluated for LC3, beclin-1, and mTOR expression by qPCR and immunohistochemistry. The mRNA for LC3 and beclin-1 were significantly lower, while mTOR gene expression was significantly higher in the placenta of pregnant women with PE than in the NT group. Placentas of PE women showed significantly decreased protein expression of LC3-II and beclin-1, whereas mTOR was significantly increased compared with the NT pregnant women. There was a negative correlation between protein expression of mTOR and LC3-II in the placental tissue of PE women. In conclusion, the results showed autophagy deficiency suggesting that failure in this degradation process may contribute to the pathogenesis of PE; however, new studies involving cross-talk between autophagy and inflammatory molecular mechanisms might help to better understand the autophagy process in this obstetric pathology.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.55 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100683en10.1590/1414-431x2022e12283
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Weel,I.C.
Ribeiro,V.R.
Romão-Veiga,M.
Fioratti,E.G.
Peraçoli,J.C.
Peraçoli,M.T.S.
spellingShingle Weel,I.C.
Ribeiro,V.R.
Romão-Veiga,M.
Fioratti,E.G.
Peraçoli,J.C.
Peraçoli,M.T.S.
Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia
author_facet Weel,I.C.
Ribeiro,V.R.
Romão-Veiga,M.
Fioratti,E.G.
Peraçoli,J.C.
Peraçoli,M.T.S.
author_sort Weel,I.C.
title Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia
title_short Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia
title_full Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia
title_fullStr Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia
title_full_unstemmed Down-regulation of autophagy proteins is associated with higher mTOR expression in the placenta of pregnant women with preeclampsia
title_sort down-regulation of autophagy proteins is associated with higher mtor expression in the placenta of pregnant women with preeclampsia
description Autophagy is a lysosomal degradation pathway that removes protein aggregates and damaged organelles maintaining cellular integrity. It seems to be essential for cell survival during stress, starvation, hypoxia, and consequently to the placenta implantation and development. Preeclampsia (PE) is a multisystemic disorder characterized by the onset of hypertension associated or not with proteinuria and other maternal complications. Considering that the placenta seems to play an important role in the pathogenesis of PE, the objective of the present study was to evaluate protein levels of light chain protein (LC3), beclin-1, and the mammalian target of rapamycin (mTOR) in the placenta of pregnant women with PE. Placental tissues collected from 20 women with PE and 20 normotensive (NT) pregnant women were evaluated for LC3, beclin-1, and mTOR expression by qPCR and immunohistochemistry. The mRNA for LC3 and beclin-1 were significantly lower, while mTOR gene expression was significantly higher in the placenta of pregnant women with PE than in the NT group. Placentas of PE women showed significantly decreased protein expression of LC3-II and beclin-1, whereas mTOR was significantly increased compared with the NT pregnant women. There was a negative correlation between protein expression of mTOR and LC3-II in the placental tissue of PE women. In conclusion, the results showed autophagy deficiency suggesting that failure in this degradation process may contribute to the pathogenesis of PE; however, new studies involving cross-talk between autophagy and inflammatory molecular mechanisms might help to better understand the autophagy process in this obstetric pathology.
publisher Associação Brasileira de Divulgação Científica
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100683
work_keys_str_mv AT weelic downregulationofautophagyproteinsisassociatedwithhighermtorexpressionintheplacentaofpregnantwomenwithpreeclampsia
AT ribeirovr downregulationofautophagyproteinsisassociatedwithhighermtorexpressionintheplacentaofpregnantwomenwithpreeclampsia
AT romaoveigam downregulationofautophagyproteinsisassociatedwithhighermtorexpressionintheplacentaofpregnantwomenwithpreeclampsia
AT fiorattieg downregulationofautophagyproteinsisassociatedwithhighermtorexpressionintheplacentaofpregnantwomenwithpreeclampsia
AT peracolijc downregulationofautophagyproteinsisassociatedwithhighermtorexpressionintheplacentaofpregnantwomenwithpreeclampsia
AT peracolimts downregulationofautophagyproteinsisassociatedwithhighermtorexpressionintheplacentaofpregnantwomenwithpreeclampsia
_version_ 1756472410440204289

Similar Items