Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats
Diabetes mellitus is associated with neural and micro- and macrovascular complications. Therapeutic options for these complications are limited and the delivery of mesenchymal stem cells into lesions have been reported to improve the healing process. In this work, the effects of the administration of a lineage of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase (hBMSC-TERT) as a potential therapeutic tool for wound healing in diabetic rats were investigated. This is the first description of the use of these cells in diabetic wounds. Dorsal cutaneous lesions were made in streptozotocin-induced diabetic rats and hBMSC-TERT were subcutaneously administered around the lesions. The healing process was evaluated macroscopically, histologically, and by birefringence analysis. Diabetic wounded rats infused with hBMSC-TERT (DM-TERT group) and the non-diabetic wounded rats not infused with hBMSC-TERT (CW group) had very similar patterns of fibroblastic response and collagen proliferation indicating improvement of wound healing. The result obtained by birefringence analysis was in accordance with that obtained by the histological analysis. The results indicated that local administration of hBMSC-TERT in diabetic wounds improved the wound healing process and may become a therapeutic option for wounds in individuals with diabetes.
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Associação Brasileira de Divulgação Científica
2021
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oai:scielo:S0100-879X20210011006062021-09-01Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic ratsLuna,G.L. FloresOehlmeyer,T.L.Brandão,G.Brassolatti,P.Tosta,J.Goto,L.S.Avó,L. deLeal,A.M. de Oliveira Cell therapy Diabetes mellitus Mesenchymal stem cells Wound Animal model Diabetes mellitus is associated with neural and micro- and macrovascular complications. Therapeutic options for these complications are limited and the delivery of mesenchymal stem cells into lesions have been reported to improve the healing process. In this work, the effects of the administration of a lineage of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase (hBMSC-TERT) as a potential therapeutic tool for wound healing in diabetic rats were investigated. This is the first description of the use of these cells in diabetic wounds. Dorsal cutaneous lesions were made in streptozotocin-induced diabetic rats and hBMSC-TERT were subcutaneously administered around the lesions. The healing process was evaluated macroscopically, histologically, and by birefringence analysis. Diabetic wounded rats infused with hBMSC-TERT (DM-TERT group) and the non-diabetic wounded rats not infused with hBMSC-TERT (CW group) had very similar patterns of fibroblastic response and collagen proliferation indicating improvement of wound healing. The result obtained by birefringence analysis was in accordance with that obtained by the histological analysis. The results indicated that local administration of hBMSC-TERT in diabetic wounds improved the wound healing process and may become a therapeutic option for wounds in individuals with diabetes.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.54 n.11 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001100606en10.1590/1414-431x2021e11352 |
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Luna,G.L. Flores Oehlmeyer,T.L. Brandão,G. Brassolatti,P. Tosta,J. Goto,L.S. Avó,L. de Leal,A.M. de Oliveira |
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Luna,G.L. Flores Oehlmeyer,T.L. Brandão,G. Brassolatti,P. Tosta,J. Goto,L.S. Avó,L. de Leal,A.M. de Oliveira Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats |
author_facet |
Luna,G.L. Flores Oehlmeyer,T.L. Brandão,G. Brassolatti,P. Tosta,J. Goto,L.S. Avó,L. de Leal,A.M. de Oliveira |
author_sort |
Luna,G.L. Flores |
title |
Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats |
title_short |
Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats |
title_full |
Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats |
title_fullStr |
Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats |
title_full_unstemmed |
Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats |
title_sort |
use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats |
description |
Diabetes mellitus is associated with neural and micro- and macrovascular complications. Therapeutic options for these complications are limited and the delivery of mesenchymal stem cells into lesions have been reported to improve the healing process. In this work, the effects of the administration of a lineage of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase (hBMSC-TERT) as a potential therapeutic tool for wound healing in diabetic rats were investigated. This is the first description of the use of these cells in diabetic wounds. Dorsal cutaneous lesions were made in streptozotocin-induced diabetic rats and hBMSC-TERT were subcutaneously administered around the lesions. The healing process was evaluated macroscopically, histologically, and by birefringence analysis. Diabetic wounded rats infused with hBMSC-TERT (DM-TERT group) and the non-diabetic wounded rats not infused with hBMSC-TERT (CW group) had very similar patterns of fibroblastic response and collagen proliferation indicating improvement of wound healing. The result obtained by birefringence analysis was in accordance with that obtained by the histological analysis. The results indicated that local administration of hBMSC-TERT in diabetic wounds improved the wound healing process and may become a therapeutic option for wounds in individuals with diabetes. |
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Associação Brasileira de Divulgação Científica |
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2021 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001100606 |
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