A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

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It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.

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Main Authors: Souza,T.F.G., Pierdoná,T.M., Macedo,F.S., Aquino,P.E.A., Rangel,G.F.P., Duarte,R.S., Silva,L.M.A., Viana,G.S.B., Alves,A.P.N.N., Montenegro,R.C., Wilke,D.V., Silveira,E.R., Alencar,N.M.N.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000900601
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spelling oai:scielo:S0100-879X20210009006012021-05-28A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound modelSouza,T.F.G.Pierdoná,T.M.Macedo,F.S.Aquino,P.E.A.Rangel,G.F.P.Duarte,R.S.Silva,L.M.A.Viana,G.S.B.Alves,A.P.N.N.Montenegro,R.C.Wilke,D.V.Silveira,E.R.Alencar,N.M.N. Sideroxylon obtusifolium (Roem. & Schult.) Methanol fraction Human keratinocytes Wound healing Burn wounds It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.54 n.9 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000900601en10.1590/1414-431x2021e10700
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Souza,T.F.G.
Pierdoná,T.M.
Macedo,F.S.
Aquino,P.E.A.
Rangel,G.F.P.
Duarte,R.S.
Silva,L.M.A.
Viana,G.S.B.
Alves,A.P.N.N.
Montenegro,R.C.
Wilke,D.V.
Silveira,E.R.
Alencar,N.M.N.
spellingShingle Souza,T.F.G.
Pierdoná,T.M.
Macedo,F.S.
Aquino,P.E.A.
Rangel,G.F.P.
Duarte,R.S.
Silva,L.M.A.
Viana,G.S.B.
Alves,A.P.N.N.
Montenegro,R.C.
Wilke,D.V.
Silveira,E.R.
Alencar,N.M.N.
A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
author_facet Souza,T.F.G.
Pierdoná,T.M.
Macedo,F.S.
Aquino,P.E.A.
Rangel,G.F.P.
Duarte,R.S.
Silva,L.M.A.
Viana,G.S.B.
Alves,A.P.N.N.
Montenegro,R.C.
Wilke,D.V.
Silveira,E.R.
Alencar,N.M.N.
author_sort Souza,T.F.G.
title A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
title_short A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
title_full A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
title_fullStr A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
title_full_unstemmed A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
title_sort proline derivative-enriched methanol fraction from sideroxylon obtusifolium leaves (mfsol) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
description It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.
publisher Associação Brasileira de Divulgação Científica
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000900601
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