Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats

Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats

The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.

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Main Authors: Barros,M.A.V., Andrade,E.B., Barros,R.G.N., Costa,I.K.M., Costa,I.C.L., Vitorino,G.F.A., Andrade,J.J.C., Paulino-Silva,K.M., Nogueira,V.O., de Brito Alves,J.L., Costa-Silva,J.H.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2018
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613
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spelling oai:scielo:S0100-879X20180006006132019-03-19Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar ratsBarros,M.A.V.Andrade,E.B.Barros,R.G.N.Costa,I.K.M.Costa,I.C.L.Vitorino,G.F.A.Andrade,J.J.C.Paulino-Silva,K.M.Nogueira,V.O.de Brito Alves,J.L.Costa-Silva,J.H. Development Epigenetics Fertility Gestation Intrauterine growth Nutrition The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.51 n.6 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613en10.1590/1414-431x20186602
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libraryname SciELO
language English
format Digital
author Barros,M.A.V.
Andrade,E.B.
Barros,R.G.N.
Costa,I.K.M.
Costa,I.C.L.
Vitorino,G.F.A.
Andrade,J.J.C.
Paulino-Silva,K.M.
Nogueira,V.O.
de Brito Alves,J.L.
Costa-Silva,J.H.
spellingShingle Barros,M.A.V.
Andrade,E.B.
Barros,R.G.N.
Costa,I.K.M.
Costa,I.C.L.
Vitorino,G.F.A.
Andrade,J.J.C.
Paulino-Silva,K.M.
Nogueira,V.O.
de Brito Alves,J.L.
Costa-Silva,J.H.
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
author_facet Barros,M.A.V.
Andrade,E.B.
Barros,R.G.N.
Costa,I.K.M.
Costa,I.C.L.
Vitorino,G.F.A.
Andrade,J.J.C.
Paulino-Silva,K.M.
Nogueira,V.O.
de Brito Alves,J.L.
Costa-Silva,J.H.
author_sort Barros,M.A.V.
title Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
title_short Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
title_full Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
title_fullStr Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
title_full_unstemmed Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
title_sort low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant wistar rats
description The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.
publisher Associação Brasileira de Divulgação Científica
publishDate 2018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613
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