Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus

Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus

Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin receptors by immunohistochemistry and expression of miRNAs-155 and -199 in serum and the cavernous tissue. Immunostaining for endothelin receptors was markedly higher in the A, D, and AD groups than in the C group. Moreover, a significant hypoexpression of the miRNA-199 in the corpus cavernosum tissue from the AD group was observed, compared to the C group. When analyzing the microRNA profile in blood, a significant hypoexpression of miRNA-155 in the AD group was observed compared to the C group. The miRNA-199 analysis demonstrated significant hypoexpression in D and AD groups compared to the C group. Our findings in corpus cavernosum showed downregulated miRNA-155 and miRNA-199 levels associated with upregulated protein expression and unaltered mRNA expression of ET receptors suggesting decreased ET receptor turnover, which can contribute to erectile dysfunction in diabetic rats exposed to high alcohol levels.

Saved in:
Bibliographic Details
Main Authors: Gonçalves,F.Z., Lizarte Neto,F.S., Novais,P.C., Gattas,D., Lourenço,L.G., de Carvalho,C.A.M., Tirapelli,D.P.C., Molina,C.A.F., Tirapelli,L.F., Tucci Jr,S.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2018
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300615
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0100-879X2018000300615
record_format ojs
spelling oai:scielo:S0100-879X20180003006152019-03-19Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitusGonçalves,F.Z.Lizarte Neto,F.S.Novais,P.C.Gattas,D.Lourenço,L.G.de Carvalho,C.A.M.Tirapelli,D.P.C.Molina,C.A.F.Tirapelli,L.F.Tucci Jr,S. Alcoholism Corpus cavernosum miRNAs Endothelin receptors Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin receptors by immunohistochemistry and expression of miRNAs-155 and -199 in serum and the cavernous tissue. Immunostaining for endothelin receptors was markedly higher in the A, D, and AD groups than in the C group. Moreover, a significant hypoexpression of the miRNA-199 in the corpus cavernosum tissue from the AD group was observed, compared to the C group. When analyzing the microRNA profile in blood, a significant hypoexpression of miRNA-155 in the AD group was observed compared to the C group. The miRNA-199 analysis demonstrated significant hypoexpression in D and AD groups compared to the C group. Our findings in corpus cavernosum showed downregulated miRNA-155 and miRNA-199 levels associated with upregulated protein expression and unaltered mRNA expression of ET receptors suggesting decreased ET receptor turnover, which can contribute to erectile dysfunction in diabetic rats exposed to high alcohol levels.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.51 n.3 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300615en10.1590/1414-431x20176329
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Gonçalves,F.Z.
Lizarte Neto,F.S.
Novais,P.C.
Gattas,D.
Lourenço,L.G.
de Carvalho,C.A.M.
Tirapelli,D.P.C.
Molina,C.A.F.
Tirapelli,L.F.
Tucci Jr,S.
spellingShingle Gonçalves,F.Z.
Lizarte Neto,F.S.
Novais,P.C.
Gattas,D.
Lourenço,L.G.
de Carvalho,C.A.M.
Tirapelli,D.P.C.
Molina,C.A.F.
Tirapelli,L.F.
Tucci Jr,S.
Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
author_facet Gonçalves,F.Z.
Lizarte Neto,F.S.
Novais,P.C.
Gattas,D.
Lourenço,L.G.
de Carvalho,C.A.M.
Tirapelli,D.P.C.
Molina,C.A.F.
Tirapelli,L.F.
Tucci Jr,S.
author_sort Gonçalves,F.Z.
title Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
title_short Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
title_full Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
title_fullStr Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
title_full_unstemmed Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
title_sort expression profile of endothelin receptors (eta and etb) and micrornas-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
description Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin receptors by immunohistochemistry and expression of miRNAs-155 and -199 in serum and the cavernous tissue. Immunostaining for endothelin receptors was markedly higher in the A, D, and AD groups than in the C group. Moreover, a significant hypoexpression of the miRNA-199 in the corpus cavernosum tissue from the AD group was observed, compared to the C group. When analyzing the microRNA profile in blood, a significant hypoexpression of miRNA-155 in the AD group was observed compared to the C group. The miRNA-199 analysis demonstrated significant hypoexpression in D and AD groups compared to the C group. Our findings in corpus cavernosum showed downregulated miRNA-155 and miRNA-199 levels associated with upregulated protein expression and unaltered mRNA expression of ET receptors suggesting decreased ET receptor turnover, which can contribute to erectile dysfunction in diabetic rats exposed to high alcohol levels.
publisher Associação Brasileira de Divulgação Científica
publishDate 2018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300615
work_keys_str_mv AT goncalvesfz expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT lizartenetofs expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT novaispc expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT gattasd expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT lourencolg expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT decarvalhocam expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT tirapellidpc expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT molinacaf expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT tirapellilf expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
AT tuccijrs expressionprofileofendothelinreceptorsetaandetbandmicrornas155and199inthecorpuscavernosumofratssubmittedtochronicalcoholismanddiabetesmellitus
_version_ 1756391559003111424

Similar Items