Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy
Colon cancer is one of the most common digestive tumors. The present study aimed to explore the functional role, as well as the underlying mechanism of long non-coding RNA LINC00261 in colon cancer. Expression of LINC00261 was analyzed in colon cancer cell lines and human normal cell lines. Acquired resistance cell lines were then built and the acquired resistance efficiency was detected by evaluating cell viability. Thereafter, the effects of LINC00261 overexpression on cisplatin-resistant colon cancer cells were measured, as well as cell apoptosis, viability, migration, and invasion. Subsequently, we investigated the interaction of LINC00261 and β-catenin. The results showed that the LINC00261 gene was down-regulated in colon cancer cell lines and tissues, and in cisplatin-resistant cells. LINC00261 overexpression might relieve cisplatin resistance of colon cancer cells via promoting cell apoptosis, and inhibiting cell viability, migration, and invasion. Moreover, LINC00261 might down-regulate nuclear β-catenin through restraining β-catenin from cytoplasm into nuclei or it could also promote β-catenin degradation and inhibit activation of Wnt pathway. Finally, LINC00261 reduced cisplatin resistance of colon cancer in vivo and enhanced the anti-colon cancer effect of cisplatin through reducing tumor volume and weight.
Main Authors: | , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
2018
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200607 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0100-879X2018000200607 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0100-879X20180002006072019-03-19Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapyWang,Z.K.Yang,L.Wu,L.L.Mao,H.Zhou,Y.H.Zhang,P.F.Dai,G.H. Colon cancer LINC00261 β-catenin Cisplatin resistance Mechanism Colon cancer is one of the most common digestive tumors. The present study aimed to explore the functional role, as well as the underlying mechanism of long non-coding RNA LINC00261 in colon cancer. Expression of LINC00261 was analyzed in colon cancer cell lines and human normal cell lines. Acquired resistance cell lines were then built and the acquired resistance efficiency was detected by evaluating cell viability. Thereafter, the effects of LINC00261 overexpression on cisplatin-resistant colon cancer cells were measured, as well as cell apoptosis, viability, migration, and invasion. Subsequently, we investigated the interaction of LINC00261 and β-catenin. The results showed that the LINC00261 gene was down-regulated in colon cancer cell lines and tissues, and in cisplatin-resistant cells. LINC00261 overexpression might relieve cisplatin resistance of colon cancer cells via promoting cell apoptosis, and inhibiting cell viability, migration, and invasion. Moreover, LINC00261 might down-regulate nuclear β-catenin through restraining β-catenin from cytoplasm into nuclei or it could also promote β-catenin degradation and inhibit activation of Wnt pathway. Finally, LINC00261 reduced cisplatin resistance of colon cancer in vivo and enhanced the anti-colon cancer effect of cisplatin through reducing tumor volume and weight.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.51 n.2 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200607en10.1590/1414-431x20176793 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Wang,Z.K. Yang,L. Wu,L.L. Mao,H. Zhou,Y.H. Zhang,P.F. Dai,G.H. |
spellingShingle |
Wang,Z.K. Yang,L. Wu,L.L. Mao,H. Zhou,Y.H. Zhang,P.F. Dai,G.H. Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy |
author_facet |
Wang,Z.K. Yang,L. Wu,L.L. Mao,H. Zhou,Y.H. Zhang,P.F. Dai,G.H. |
author_sort |
Wang,Z.K. |
title |
Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy |
title_short |
Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy |
title_full |
Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy |
title_fullStr |
Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy |
title_full_unstemmed |
Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy |
title_sort |
long non-coding rna linc00261 sensitizes human colon cancer cells to cisplatin therapy |
description |
Colon cancer is one of the most common digestive tumors. The present study aimed to explore the functional role, as well as the underlying mechanism of long non-coding RNA LINC00261 in colon cancer. Expression of LINC00261 was analyzed in colon cancer cell lines and human normal cell lines. Acquired resistance cell lines were then built and the acquired resistance efficiency was detected by evaluating cell viability. Thereafter, the effects of LINC00261 overexpression on cisplatin-resistant colon cancer cells were measured, as well as cell apoptosis, viability, migration, and invasion. Subsequently, we investigated the interaction of LINC00261 and β-catenin. The results showed that the LINC00261 gene was down-regulated in colon cancer cell lines and tissues, and in cisplatin-resistant cells. LINC00261 overexpression might relieve cisplatin resistance of colon cancer cells via promoting cell apoptosis, and inhibiting cell viability, migration, and invasion. Moreover, LINC00261 might down-regulate nuclear β-catenin through restraining β-catenin from cytoplasm into nuclei or it could also promote β-catenin degradation and inhibit activation of Wnt pathway. Finally, LINC00261 reduced cisplatin resistance of colon cancer in vivo and enhanced the anti-colon cancer effect of cisplatin through reducing tumor volume and weight. |
publisher |
Associação Brasileira de Divulgação Científica |
publishDate |
2018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200607 |
work_keys_str_mv |
AT wangzk longnoncodingrnalinc00261sensitizeshumancoloncancercellstocisplatintherapy AT yangl longnoncodingrnalinc00261sensitizeshumancoloncancercellstocisplatintherapy AT wull longnoncodingrnalinc00261sensitizeshumancoloncancercellstocisplatintherapy AT maoh longnoncodingrnalinc00261sensitizeshumancoloncancercellstocisplatintherapy AT zhouyh longnoncodingrnalinc00261sensitizeshumancoloncancercellstocisplatintherapy AT zhangpf longnoncodingrnalinc00261sensitizeshumancoloncancercellstocisplatintherapy AT daigh longnoncodingrnalinc00261sensitizeshumancoloncancercellstocisplatintherapy |
_version_ |
1756391555145400320 |