Typing class I HLA-A gene using a nested PCR-RFLP procedure

Typing class I HLA-A gene using a nested PCR-RFLP procedure

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.

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Main Authors: Castelli,E.C., Gil,D.S., Veiga,L.C.S., de Camargo,J.L.V.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2005
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004
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spelling oai:scielo:S0100-879X20050006000042005-09-28Typing class I HLA-A gene using a nested PCR-RFLP procedureCastelli,E.C.Gil,D.S.Veiga,L.C.S.de Camargo,J.L.V. Human leukocyte antigen-A HLA-A Typing RFLP Polymorphism Major histocompatibility complex In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.38 n.6 20052005-06-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004en10.1590/S0100-879X2005000600004
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Castelli,E.C.
Gil,D.S.
Veiga,L.C.S.
de Camargo,J.L.V.
spellingShingle Castelli,E.C.
Gil,D.S.
Veiga,L.C.S.
de Camargo,J.L.V.
Typing class I HLA-A gene using a nested PCR-RFLP procedure
author_facet Castelli,E.C.
Gil,D.S.
Veiga,L.C.S.
de Camargo,J.L.V.
author_sort Castelli,E.C.
title Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_short Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_full Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_fullStr Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_full_unstemmed Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_sort typing class i hla-a gene using a nested pcr-rflp procedure
description In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
publisher Associação Brasileira de Divulgação Científica
publishDate 2005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004
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