Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum

Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum

Dopamine nigrostriatal neurons are important for motor control and may contain a particularly dense population of ryanodine receptors involved in the control of dopamine release. To test this hypothesis, we used a classical model of unilateral selective lesion of these neurons in rats based on 6-hydroxydopamine (6-OHDA) injection into the substantia nigra. Binding of [3H]-GBR 12935, used as a presynaptic marker since it labels specifically the dopamine uptake complex, was dramatically decreased by 83-100% in striatum homogenates after 6-OHDA lesion. On the contrary, no reduction of [3H]-ryanodine binding was observed. The present data indicate that [3H]-ryanodine binding sites present in rat striatum are not preferentially localized in dopaminergic terminals.

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Main Authors: Noël,F., Geurts,M., Maloteaux,J.-M.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2000
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200013
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spelling oai:scielo:S0100-879X20000002000132000-02-02Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatumNoël,F.Geurts,M.Maloteaux,J.-M. ryanodine striatum 6-hydroxydopamine dopamine neurons neuroleptic malignant syndrome Dopamine nigrostriatal neurons are important for motor control and may contain a particularly dense population of ryanodine receptors involved in the control of dopamine release. To test this hypothesis, we used a classical model of unilateral selective lesion of these neurons in rats based on 6-hydroxydopamine (6-OHDA) injection into the substantia nigra. Binding of [3H]-GBR 12935, used as a presynaptic marker since it labels specifically the dopamine uptake complex, was dramatically decreased by 83-100% in striatum homogenates after 6-OHDA lesion. On the contrary, no reduction of [3H]-ryanodine binding was observed. The present data indicate that [3H]-ryanodine binding sites present in rat striatum are not preferentially localized in dopaminergic terminals.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.33 n.2 20002000-02-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200013en10.1590/S0100-879X2000000200013
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Noël,F.
Geurts,M.
Maloteaux,J.-M.
spellingShingle Noël,F.
Geurts,M.
Maloteaux,J.-M.
Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
author_facet Noël,F.
Geurts,M.
Maloteaux,J.-M.
author_sort Noël,F.
title Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
title_short Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
title_full Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
title_fullStr Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
title_full_unstemmed Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
title_sort selective destruction of nigrostriatal dopaminergic neurons does not alter [3h]-ryanodine binding in rat striatum
description Dopamine nigrostriatal neurons are important for motor control and may contain a particularly dense population of ryanodine receptors involved in the control of dopamine release. To test this hypothesis, we used a classical model of unilateral selective lesion of these neurons in rats based on 6-hydroxydopamine (6-OHDA) injection into the substantia nigra. Binding of [3H]-GBR 12935, used as a presynaptic marker since it labels specifically the dopamine uptake complex, was dramatically decreased by 83-100% in striatum homogenates after 6-OHDA lesion. On the contrary, no reduction of [3H]-ryanodine binding was observed. The present data indicate that [3H]-ryanodine binding sites present in rat striatum are not preferentially localized in dopaminergic terminals.
publisher Associação Brasileira de Divulgação Científica
publishDate 2000
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200013
work_keys_str_mv AT noelf selectivedestructionofnigrostriataldopaminergicneuronsdoesnotalter3hryanodinebindinginratstriatum
AT geurtsm selectivedestructionofnigrostriataldopaminergicneuronsdoesnotalter3hryanodinebindinginratstriatum
AT maloteauxjm selectivedestructionofnigrostriataldopaminergicneuronsdoesnotalter3hryanodinebindinginratstriatum
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